Investigation of metabolism using TLC-DAR and reaction-displacement TLC

被引：10
作者：
Kalász, H
Lengyel, J
Szarvas, T
Morovján, G
Klebovich, I
机构：
[1] Semmelweis Univ, Dept Pharmacol & Pharmacotherapy, H-1089 Budapest, Hungary
[2] Semmelweis Univ, Cent Isotope Lab, H-1089 Budapest, Hungary
[3] EGIS Pharmaceut Co Ltd, Dept Pharmacokinet, H-1106 Budapest, Hungary
来源：
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2003年 / 16卷 / 05期
关键词：
thin-layer chromatography-digital autoradlography; formaldehyde; formaldemedone; metabolism; N-demethylation; (-)-deprenyl;
D O I：
10.1556/JPC.16.2003.5.10
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Thin-layer chromatography has been used to monitor formaldehyde production during in-vivo metabolism of (-)-deprenyl. Formaldehyde was reacted with dimedone to yield its dimedone adduct, formaldemedone. Determination of C-14-labeled formaldemedone was performed by planar displacement chromatography. The separated bands were detected either by digital autoradiography or on X-ray film after contact autoradiography. Two-dimensional separations have improved the evaluation.
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页码：381 / 385
页数：5
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