Enhanced Enzymatic Saccharification of Tomato Stalk by Combination Pretreatment with NaOH and ChCl:Urea-Thioure in One-Pot Manner

In this study, the mixture of NaOH and deep eutectic solvent (DES) ChCl:UA-TA was firstly used to pretreat waste tomato stalk (TS). The effects of pretreatment time, pretreatment temperature, NaOH dosage, and DES dose were investigated, and the synergistic effects of dilute NaOH and DES combination pretreatment were tested on the influence of enzymatic saccharification. It was found that the relationship between delignification and saccharification rate had a significant linear correction. When TS was pretreated with NaOH (7 wt%)-ChCl:UA-TA (8 wt%) in a solid-to-liquid ratio of 1:10 (wt:wt) at 75 degrees C for 60 min, the delignification reached 82.1%. The highest yield of reducing sugars from NaOH-ChCl:UA-TA-treated TS could reach 62.5% in an acetate buffer (50 mM, pH 4.8) system containing cellulase (10.0 FPU/g TS) and xylanase (30.0 CBU/g TS) at 50 degrees C. In summary, effective enzymatic saccharification of TS was developed by a combination pretreatment with dilute NaOH and ChCl:UA-TA, which has potential application in the future.