Effects of single and multi walled carbon nanotubes on macrophages: Cyto and genotoxicity and electron microscopy

被引：139

作者：

Di Giorgio, Maria Laura ^{[1
]}

Di Bucchianico, Sebastiano ^{[1
]}

Ragnelli, Anna Maria ^{[1
]}

Aimola, Pierpaolo ^{[1
]}

Santucci, Sandro ^{[2
]}

Poma, Anna ^{[1
]}

机构：

[1] Univ Aquila, Dept Basic & Appl Biol, I-67010 Coppito, Italy

[2] Univ Aquila, Dept Phys, I-67100 Laquila, Italy

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2011年 / 722卷 / 01期

关键词：

Carbon nanotubes genotoxicity; Carbon black; Transmission electron microscopy; Scanning electron microscopy; Atomic force microscopy; Reactive oxygen species; OXIDATIVE STRESS; ULTRAFINE PARTICLES; PULMONARY TOXICITY; DNA-DAMAGE; CYTOTOXICITY; CELLS; NANOMATERIALS; FULLERENES; MEMBRANES; ASBESTOS;

D O I：

10.1016/j.mrgentox.2011.02.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Production of nanotechnology-based materials is increasing worldwide: it is essential to evaluate their potential toxicity. Among these nanomaterials, carbon nanotubes (CNTs) have tremendous potential in many areas of research and applications. We have investigated the cyto- and genotoxic effects of single and multi-walled CNTs (SWCNTs, MWCNTs) and carbon black (CB) on the mouse macrophage cell line RAW 264.7. Specifically we have investigated inflammatory response, release of tumor necrosis factor-a (TNF-alpha), intracellular reactive oxygen species (ROS) production, cell death (both necrosis and apoptosis), chromosomal aberrations and cellular ultrastructural alteration caused by CB. MWCNTs and SWCNTs. Our data confirm that both CNTs and CB are cyto and geno-toxic to RAW 264.7 mouse macrophages. CNTs exposure induced ROS release, necrosis and chromosomal aberrations but did not cause an inflammatory response. In addition CNTs induce ultrastructural damage and apoptosis. CNTs penetrate the cell membrane and individual MWCNTs are seen associated with the nuclear envelope. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：20 / 31

页数：12

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