Generation of a DAPK1 knockout first (conditional ready) human embryonic stem cell line (ZSSYe001-A) by CRISPR-Cas9 technology

被引：3
|
作者：
Xu, Cancan [2 ,3 ]
Zhou, Zhuowei [1 ]
Liu, Chang [1 ]
Kang, Xinmei [1 ]
Zhong, Xiaofen [3 ]
Zhang, Qi [1 ]
Xu, Yan [1 ]
机构：
[1] Sun Yat Sen Univ, Affiliated Hosp 3, Biotherapy Ctr, Guangzhou 510630, Peoples R China
[2] Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Peoples R China
[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Stem Cell & Regenerat Med, CAS Key Lab Regenerat Biol, Guangzhou 510530, Peoples R China
来源：
STEM CELL RESEARCH | 2020年 / 43卷
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.scr.2019.101693
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Death-associated protein kinase 1 (DAPK1) is a Ca2+/calmodulin regulated Ser/Thr kinase involved in various cellular processes including cell death, autophagy and inflammation. Its dysregulation has been linked to tumour metastasis, anti-viral responses, Alzheimer's disease and other neurological disorders. To further investigate the role of DAPK1 in these processes, we generated a DAPK1 knockout first (conditional ready) human embryonic stem (hES) cell line in which the endogenous DAPK1 can be easily restored with expression of FLPe. This cell line provides an ideal model to study the role of DAPK1 in human development and various pathologies related to DAPK1 dysregulation in vitro.
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页数：5
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