Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

被引：15

作者：

Nguyen Duc Huy ^{[1
]}

Kim, Seung-Wook ^{[2
]}

Park, Seung-Moon ^{[1
]}

机构：

[1] Chonbuk Natl Univ, Coll Environm & Bioresource Sci, Div Biotechnol, Iksan 570752, South Korea

[2] Korea Univ, Dept Chem & Biol Engn, Seoul 136701, South Korea

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2011年 / 111卷 / 06期

关键词：

Phanerochaete chrysosporium; Pichia pastoris; Xylan; Xylanase; Glycoside hydrolase; PROTEIN; GENE; XYLANASES; CLONING;

D O I：

10.1016/j.jbiosc.2011.02.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The cDNA of endo-1,4-beta-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the alpha-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500 U.I-1. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：654 / 657

页数：4

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