The phenanthroimidazole-based dizinc(II) complex as a fluorescent probe for the pyrophosphate ion as generated in polymerase chain reactions and pyrosequencing

被引:19
作者
Anbu, Sellamuthu [1 ]
Kamalraj, Subban [2 ]
Paul, Anup [1 ]
Jayabaskaran, Chelliah [2 ]
Pombeiro, Armando J. L. [1 ]
机构
[1] Univ Lisbon, Ctr Quim Estrutural, Inst Super Tecn, Complexo 1,Av Rovisco Pais, P-1049001 Lisbon, Portugal
[2] Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India
关键词
SELECTIVE RECOGNITION; PHOSPHATE; CHEMOSENSOR; DESIGN; ATP; SENSORS; ANIONS; COORDINATION; RECEPTORS; BINDING;
D O I
10.1039/c4dt03590a
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
A highly selective and sensitive phenanthroimidazole tagged Mannich base type dizinc(II) fluorescent probe (R-Zn2+) has been developed for the pyrophosphate ion (PPi) with a very low limit of detection (LOD) of 0.25 ppm; this also assesses PPi from DNA polymerization chain reaction (PCR).
引用
收藏
页码:3930 / 3933
页数:4
相关论文
共 51 条
[1]   Differentially Selective Chemosensor with Fluorescence Off-On Responses on Cu2+ and Zn2+ Ions in Aqueous Media and Applications in Pyrophosphate Sensing, Live Cell Imaging, and Cytotoxicity [J].
Anbu, Sellamuthu ;
Ravishankaran, Rajendran ;
Guedes da Silva, M. Fatima C. ;
Karande, Anjali A. ;
Pombeiro, Armando J. L. .
INORGANIC CHEMISTRY, 2014, 53 (13) :6655-6664
[2]   Naphthalene Carbohydrazone Based Dizinc(II) Chemosensor for a Pyrophosphate Ion and Its DNA Assessment Application in Polymerase Chain Reaction Products [J].
Anbu, Sellamuthu ;
Kamalraj, Subban ;
Jayabaskaran, Chelliah ;
Mukherjee, Partha Sarathi .
INORGANIC CHEMISTRY, 2013, 52 (15) :8294-8296
[3]   A Highly Selective Pyrophosphate Sensor Based on ESIPT Turn-On in Water [J].
Chen, Wei-Hua ;
Xing, Yu ;
Pang, Yi .
ORGANIC LETTERS, 2011, 13 (06) :1362-1365
[4]  
Diamond D., 1996, CHEM SOC REV, V15
[5]  
Fabbrizzi L, 2002, ANGEW CHEM INT EDIT, V41, P3811, DOI 10.1002/1521-3773(20021018)41:20<3811::AID-ANIE3811>3.0.CO
[6]  
2-W
[7]   The design of luminescent sensors for anions and ionisable analytes [J].
Fabbrizzi, L ;
Licchelli, M ;
Rabaioli, G ;
Taglietti, A .
COORDINATION CHEMISTRY REVIEWS, 2000, 205 :85-108
[8]   Structural and molecular recognition studies with acyclic anion receptors [J].
Gale, Philip A. .
ACCOUNTS OF CHEMICAL RESEARCH, 2006, 39 (07) :465-475
[9]   Fluorine NMR reporter for phosphate anions [J].
Gan, Haiying ;
Oliver, Allen G. ;
Smith, Bradley D. .
CHEMICAL COMMUNICATIONS, 2013, 49 (44) :5070-5072
[10]   A highly selective fluorescent probe for pyrophosphate detection in aqueous solutions [J].
Ganjali, M. R. ;
Hosseini, M. ;
Aboufazeli, F. ;
Faridbod, F. ;
Goldooz, H. ;
Badiei, A. R. .
LUMINESCENCE, 2012, 27 (01) :20-23