A comparative insight into the mechanical properties, antibacterial potential, and cytotoxicity profile of nano-hydroxyapatite and nano-whitlockite-incorporated poly-L-lactic acid for bone tissue engineering

被引:7
|
作者
Nazir, Farzana [1 ]
Abbas, Laraib [1 ]
Iqbal, Mudassir [1 ]
机构
[1] Natl Univ Sci & Technol NUST, Sch Nat Sci SNS, Dept Chem, Islamabad 44000, Pakistan
关键词
Poly-l-lactide; Hydroxyapatite; Whitlockite; Mechanical properties; Bone tissue engineering; ALGINATE-GELATIN MICROCAPSULE; IN-VITRO DEGRADATION; HYDROLYTIC DEGRADATION; POLY(LACTIC ACID); OSTEOGENIC DIFFERENTIATION; STRUCTURAL-CHANGES; PLLA; SCAFFOLDS; FABRICATION; COMPOSITES;
D O I
10.1007/s13204-021-02223-6
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
This work reports the synthesis and characterization of nanohydroxyapatite (nHA) and nanowhitlockite (nWH), which were further incorporated to poly-l-lactic acid (PLLA) to study their effects on the structure, mechanical properties, antibacterial potential, and cytotoxicity of PLLA. SEM images of PLLA-nHA and PLLA-nWH revealed uniform dispersion of nanoparticles in polymer. FTIR spectroscopy showed shift in functional group position and indicated transformation of PLLA alpha ' to more crystalline PLLA alpha, which was further confirmed by X-ray diffraction. The mechanical properties of the materials showed that addition of nanoparticles have made PLLA ductile and strong pertaining to the effective dissipation of loads across the interfaces, enabling them to delay the crack growth by avoiding stress concentration sites. PLLA-nWH10% showed the highest antibacterial activity against Gram-positive bacteria Listeria monocytogenes and Staphylococcus aureus. In vitro cytocompatibility of PLLA-nWH10% was highest, 98%, at day 7 against fibroblasts MC3T3-E1 (osteoblastic cell line), whereas all nanocomposites have enhanced cell viability (94%) as compared to pure PLLA. Alizarin red assay showed twofold increase in calcification at day 14 with PLLA-nWH as compared to PLLA-nHA. It was clearly observed that binary system PLLA-nWH at all concentrations had good dispersion and interfacial interaction resulting in improved mechanical and enhanced osteoconductive properties as compared to PLLA-nHA.
引用
收藏
页码:47 / 68
页数:22
相关论文
共 29 条
  • [21] Design and evaluation of poly (lactic-co-glyclic acid)/poly (vinyl alcohol)/nano-hydroxyapatite hydrogels for cartilage tissue engineering in vitro
    Zeng, Min
    Xie, Jie
    Li, Mingqing
    Lin, Shaoru
    Su, Weiping
    Hu, Yihe
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2016, 9 (06): : 9817 - 9827
  • [22] Needle-like nano hydroxyapatite/poly(L-lactide acid) composite scaffold for bone tissue engineering application
    Nejati, E.
    Firouzdor, V.
    Eslaminejad, M. B.
    Bagheri, F.
    MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2009, 29 (03): : 942 - 949
  • [23] Enhanced Mechanical Properties and Degradation Control of Poly(Lactic) Acid/Hydroxyapatite/Reduced Graphene Oxide Composites for Advanced Bone Tissue Engineering Application
    Omigbodun, Francis T.
    Oladapo, Bankole I.
    BIOMIMETICS, 2024, 9 (11)
  • [24] Three-dimensional printed poly(L-lactide) copolymers with nano-hydroxyapatite scaffolds for enhanced osteogenic and regenerative activities in bone tissue engineering
    Fan, Tiantang
    Zhang, Yujue
    Meng, Xiao
    Qu, Yangcui
    Wang, Ying
    Liu, Qing
    Wang, Guannan
    COLLOID AND INTERFACE SCIENCE COMMUNICATIONS, 2022, 51
  • [25] Fabrication and characterization of novel ethyl cellulose-grafted-poly (e-caprolactone)/alginate nanofibrous/macroporous scaffolds incorporated with nano-hydroxyapatite for bone tissue engineering
    Hokmabad, Vahideh Raeisdasteh
    Davaran, Soodabeh
    Aghazadeh, Marziyeh
    Rahbarghazi, Reza
    Salehi, Roya
    Ramazani, Ali
    JOURNAL OF BIOMATERIALS APPLICATIONS, 2019, 33 (08) : 1128 - 1144
  • [26] Facile preparation and properties of porous poly(vinyl alcohol)/trehalose/ nano-clay hydrogels with high mechanical strength for potential application in bone tissue engineering
    Xiang, Changxin
    Zhang, Qing
    Guo, Zijian
    Wang, Zehua
    Chen, Weiyi
    Li, Xiaona
    Wei, Xiaochun
    Li, Pengcui
    EUROPEAN POLYMER JOURNAL, 2023, 197
  • [27] Bone Regeneration with BMP-2 Gene-modified Mesenchymal Stem Cells Seeded on Nano-hydroxyapatite/Collagen/Poly(L-Lactic Acid) Scaffolds
    Dong, Jin-Lei
    Li, Lian-Xin
    Mu, Wei-Dong
    Wang, Yong-Hui
    Zhou, Dong-Sheng
    Hao, Wei
    Zou, De-Bo
    Hu, Kun
    Li, Ji
    Cui, Fu-Zhai
    JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, 2010, 25 (06) : 547 - 566
  • [28] The effect of polyethylene glycol on printability, physical and mechanical properties and osteogenic potential of 3D-printed poly (L-lactic acid)/ polyethylene glycol scaffold for bone tissue engineering
    Salehi, Saiedeh
    Ghomi, Hamed
    Hassanzadeh-Tabrizi, S. A.
    Koupaei, Narjes
    Khodaei, Mohammad
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, 221 : 1325 - 1334
  • [29] Electronic, mechanical, and thermal properties of zirconium dioxide nanotube interacting with poly lactic-co-glycolic acid and chitosan as potential agents in bone tissue engineering: insights from computational approaches
    Almalki, Atiah H. H.
    Belal, Amany
    Farghali, Ahmed A. A.
    Mahmoud, Rehab
    Mustafa, F. M.
    Abd El-Mageed, H. R.
    JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2024, 42 (01) : 231 - 243