The Analysis of IgG-Protein A Binding Effect by Quartz Crystal Microbalance Biosensor

被引：0

作者：

Cheng, Chi-Jung ^{[1
]}

Feng, Chen-Tung ^{[1
]}

Atashbar, Massood Z. ^{[1
]}

机构：

[1] Western Michigan Univ, Dept Elect & Comp Engn, Kalamazoo, MI 49008 USA

来源：

2010 IEEE SENSORS | 2010年

关键词：

GAS SENSOR; LIQUID; RESONATOR; LAYER;

D O I：

10.1109/ICSENS.2010.5690442

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

In this study, we present experimental, modeling and simulation results for affinity binding between human immunoglobulin G (IgG) and protein A using an AT-cut quartz crystal microbalance (QCM) biosensor. A multilayer configuration transmission line model was built to predict the IgG-Protein A binding affinity on the QCM surface. The influence of antibody-antigen bonding effect on resonance frequency was analyzed by varying the IgG concentrations. The experiments registered 440 Hz, 360 Hz, 215 Hz and 110 Hz for IgG concentrations of 100 mu g/ml, 50 mu g/ml, 10 mu g/ml and 5 mu g/ml respectively, while the simulation resulted in 480 Hz, 409 Hz, 298 Hz and 130 Hz, respectively.

引用

页码：228 / 231

页数：4

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