Photobacterium jeanii sp nov., isolated from corals and zoanthids

被引：31

作者：

Chimetto, Luciane A. ^{[1
,2
,3
]}

Cleenwerck, Ilse ^{[3
]}

Thompson, Cristiane C. ^{[4
]}

Brocchi, Marcelo ^{[2
]}

Willems, Anne ^{[5
]}

De Vos, Paul ^{[3
,5
]}

Thompson, Fabiano L. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biol, Dept Genet, Rio De Janeiro, Brazil

[2] State Univ Campinas UNICAMP, Inst Biol, Dept Genet Evolut & Bioagents, Sao Paulo, Brazil

[3] Univ Ghent, BCCM LMG Bacteria Collect, B-9000 Ghent, Belgium

[4] Fundacao Oswaldo Cruz, FIOCRUZ IOC, Microbial Genet Lab, Rio De Janeiro, Brazil

[5] Univ Ghent, Fac Sci, Microbiol Lab, B-9000 Ghent, Belgium

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2010年 / 60卷

基金：

巴西圣保罗研究基金会;

关键词：

DEOXYRIBONUCLEIC-ACID; DNA HYBRIDIZATION; GENOMIC DIVERSITY; VIBRIOS; BACTERIUM; SEA; MUCUS; DISEASE;

D O I：

10.1099/ijs.0.019968-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Four novel isolates (R-40508(T), R-40507, R-40903 and R-21419) were obtained from different cnidarian species (Phyllogorgia dilatata, Merulina ampliata and Palythoa caribaeorum) from different places in Brazil and Australia. The novel isolates formed a tight phylogenetic group based on 16S rRNA, recA, topA, ftsZ, mreB and rpoA gene sequences. Their closest phylogenetic neighbours were the type strains of Photobacterium leiognathi, P. rosenbergii and P. halotolerans, sharing 97.1-97.5% 16S rRNA gene sequence similarity. DNA-DNA hybridization between a representative strain (R-40508(T)) and the type strains of these Photobacterium species revealed less than 20% relatedness, showing that the new isolates belong to a novel species. Several phenotypic features allow the differentiation of the novel species from its closest phylogenetic neighbours. It has gelatinase and lipase activity and can utilize melibiose, but it cannot grow on 6% NaCl. In addition, the novel species has the fatty acid iso-C-16:0, but lacks the fatty acids C-17:0, C-17:0 cyclo, iso-C-17:0, C-17:1 omega Bc and iso-C-17:1 omega 9c. The name Photobacterium jeanii sp. nov. is proposed for this species, with the type strain R-40508(T) (=LMG 25436(T) =CAIM 1817(T)). The G + C content of the type strain is 45.5 mol%.

引用

页码：2843 / 2848

页数：6

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