Profiling of Host Cell Response to Successive Canine Parvovirus Infection Based on Kinetic Proteomic Change Identification

被引：3

作者：

Zhao, Hang ^{[1
]}

Cheng, Yuening ^{[1
]}

Wang, Jianke ^{[1
]}

Lin, Peng ^{[1
]}

Yi, Li ^{[1
]}

Sun, Yaru ^{[1
]}

Ren, Jingqiang ^{[1
]}

Tong, Mingwei ^{[1
]}

Cao, Zhigang ^{[1
]}

Li, Jiawei ^{[1
]}

Deng, Jinliang ^{[1
]}

Cheng, Shipeng ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Special Anim & Plant Sci, State Key Lab Mol Biol Special Econ Anim, Changchun 130112, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

TUMOR-ASSOCIATED ANTIGEN; PANDEMIC VIRUSES; ISOBARIC TAGS; PROTEINS; HSP60; REPLICATION; APOPTOSIS; ACTIVATION; INSIGHTS; SURVIVAL;

D O I：

10.1038/srep29560

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Canine parvovirus (CPV) reproduces by co-opting the resources of host cells, inevitably causing cytotoxic effects to the host cells. Feline kidney F81 cells are sensitive to CPV infection and show disparate growing statuses at different time points post-infection. This study analysed the response of F81 cells to CPV infection at successive infection time points by iTRAQ-based quantitative proteomics. Differentially expressed proteins (DEPs) during 60 h of infection and at selected time points post-infection were identified by an analysis of variance test and a two-tailed unpaired t test, respectively. DEPs with similar quantitative changes were clustered by hierarchical clustering and analysed by gene ontology enrichment, revealing that 12 h and 60 h post-infection were the optimal times to analyse the autonomous parvovirus replication and apoptosis processes, respectively. Using the Metacore (TM) database, 29 DEPs were enriched in a network involved in p53 regulation. Besides, a significantly enriched pathway suggests that the CPV-induced cytopathic effect was probably due to the deficiency of functional CFTR caused by CPV infection. This study uncovered the systemic changes in key cellular factors involved in CPV infection and help to understand the molecular mechanisms of the anti-cancer activity of CPV and the cytopathic effects induced by CPV infection.

引用

页数：11

共 56 条

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