MiR-645 promotes invasiveness, metastasis and tumor growth in colorectal cancer by targeting EFNA5

被引：31
作者：
Li, Shuai [1 ]
Hou, Xinfang [1 ]
Wu, Chen [1 ]
Han, Lili [1 ]
Li, Qian [1 ]
Wang, Jufeng [1 ]
Luo, Suxia [1 ]
机构：
[1] Zhengzhou Univ, Affiliated Canc Hosp, Henan Canc Hosp, Dept Internal Med, 127 Dongming Rd, Zhengzhou 450008, Henan, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2020年 / 125卷
关键词：
MiR-645; EFNA5; Cell invasion; Metastasis; Colorectal cancer; HEPATOCELLULAR-CARCINOMA; PROGRESSION; MIR-20A; GLIOMA;
D O I：
10.1016/j.biopha.2020.109889
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
MicroRNA-645 (miR-645) has been implicated in numerous types of human cancers including colon cancer. However, the effects and mechanisms of action of miR-645 dysregulation on the growth and malignancy of colorectal cancer (CRC) remain unclear. In this study, we demonstrated that miR-645 knockdown significantly diminished CRC cell migration and invasion and repressed epithelial-mesenchymal transition (EMT). Conversely, miR-645 overexpression enhanced CRC cell migration, invasion, and EMT. In vivo assays confirmed that miR-645 knockdown substantially reduced CRC growth and metastasis. Regarding the mechanism, ephrinA5 (EFNA5) was identified as a direct target gene of miR-645. MiR-645 specifically targeted the 3'-untranslated region of EFNA5 mRNA and hindered its expression. EFNA5 knockdown attenuated the effects of miR-645 knockdown on CRC cell migration and invasion. Additionally, we noted a statistically significant inverse correlation between EFNA5 mRNA and miR-645 levels in tumors from 28 patients with CRC. Hence, miR-645 acts as an oncogenic miRNA that may increase CRC cell migration, invasiveness, and metastasis by targeting EFNA5.
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页数：7
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