Expression, Purification and Characterization of Maltase from "Quick" Baker's Yeast

被引：1

作者：

Zhang, Cui-Ying ^{[1
]}

Song, Hai-Yan ^{[1
]}

Lin, Xue ^{[1
]}

Bai, Xiao-Wen ^{[1
]}

Xiao, Dong-Guang ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Biotechnol, Minist Educ, Tianjin Ind Microbiol Key Lab,Key Lab Ind Ferment, Tianjin 300457, Peoples R China

来源：

ADVANCES IN APPLIED BIOTECHNOLOGY, VOL II | 2015年 / 333卷

关键词：

Quick" baker's yeast; Maltase; Enzymatic properties; ALPHA-GLUCOSIDASE; SACCHAROMYCES-CEREVISIAE; LEAN DOUGH; MALTOSE; IDENTIFICATION;

D O I：

10.1007/978-3-662-46318-5_29

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The "quick" baker's yeasts are capable to rapidly metabolize maltose, thereby improving leavening ability. Given that maltase is the determining factor in maltose fermentation for "quick" baker's yeast, it is hence necessary to research the properties of the maltase independently for the "quick" baker's yeast. In this study, the heterogeneous expression of MAL62 encoding for maltase and purification of maltase were well completed. Furthermore, the enzymatic properties of maltase concentrated on the effects of substrate (maltose) and end product (glucose) were investigated as well as the physic-chemical properties and transglycosylation activity. The substrate maltose did not affect the activity of maltase, while the maltase activity was inhibited by the end product. This study provides guidance for the research of maltose metabolism for "quick" baker's yeast.

引用

页码：265 / 275

页数：11

共 50 条

[21] Functional Characterization of Extracellular Vesicles from Baker's Yeast Saccharomyces Cerevisiae as a Novel Vaccine Material for Immune Cell Maturation [J].

Higuchi, Ayaka ;

Morishita, Masaki ;

Nagata, Ryoga ;

Maruoka, Kento ;

Katsumi, Hidemasa ;

Yamamoto, Akira .

JOURNAL OF PHARMACEUTICAL SCIENCES, 2023, 112 (02) :525-534

[22] Cadmium biosorption by baker's yeast in aqueous suspensions [J].

Talos, Katalin ;

Pernyeszi, Timea ;

Majdik, Cornelia ;

Hegedusova, Alzbeta ;

Pager, Csilla .

JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 2012, 77 (04) :549-561

[23] Bread Dough and Baker's Yeast: An Uplifting Synergy [J].

Struyf, Nore ;

Van der Maelen, Eva ;

Hemdane, Sami ;

Verspreet, Joran ;

Verstrepen, Kevin J. ;

Courtin, Christophe M. .

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2017, 16 (05) :850-867

[24] Catalytic activity of baker's yeast in ester hydrolysis [J].

Bialecka-Florjanczyk, Ewa ;

Krzyczkowska, Jolanta ;

Stolarzewicz, Izabela .

BIOCATALYSIS AND BIOTRANSFORMATION, 2010, 28 (04) :288-291

[25] The Ecology and Evolution of the Baker's Yeast Saccharomyces cerevisiae [J].

Bai, Feng-Yan ;

Han, Da-Yong ;

Duan, Shou-Fu ;

Wang, Qi-Ming .

GENES, 2022, 13 (02)

[26] MATHEMATICAL MODEL OF GLYCEROL CYCLE IN BAKER'S YEAST [J].

Brusbardis, Valters ;

Liepins, Janis .

RESEARCH FOR RURAL DEVELOPMENT 2010, VOL 1, 2010, :214-+

[27] Mineralization induced by phosphorylated dry baker's yeast [J].

Ojima, Yoshihiro ;

Kihara, Maya ;

Yoshida, Mami ;

Igarashi, Koichi ;

Yoshida, Tomoko ;

Azuma, Masayuki .

PLOS ONE, 2020, 15 (09)

[28] The Effect of Different Temperatures on Autolysis of Baker's Yeast for the Production of Yeast Extract [J].

Tanguler, Hasan ;

Erten, Hueseyin .

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 2009, 33 (02) :149-154

[29] High-performance mesoporous LiFePO4 from Baker's yeast [J].

Zhang, Xudong ;

Zhang, Xueguang ;

He, Wen ;

Sun, Caiyun ;

Ma, Jingyun ;

Yuan, Junling ;

Du, Xiaoyong .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2013, 103 :114-120

[30] Activity and stability of soluble and immobilized α-glucosidase from baker's yeast in cosolvent systems [J].

Prodanovic, Radivoje ;

Milosavic, Nenad ;

Jovanovic, Slobodan ;

Prodanovic, Olivera ;

Velickovic, Tanja Cirkovic ;

Vujcic, Zoran ;

Jankov, Ratko M. .

BIOCATALYSIS AND BIOTRANSFORMATION, 2006, 24 (03) :195-200

← 1 2 3 4 5 →