Expression, Purification and Characterization of Maltase from "Quick" Baker's Yeast

被引：1

作者：

Zhang, Cui-Ying ^{[1
]}

Song, Hai-Yan ^{[1
]}

Lin, Xue ^{[1
]}

Bai, Xiao-Wen ^{[1
]}

Xiao, Dong-Guang ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Biotechnol, Minist Educ, Tianjin Ind Microbiol Key Lab,Key Lab Ind Ferment, Tianjin 300457, Peoples R China

来源：

ADVANCES IN APPLIED BIOTECHNOLOGY, VOL II | 2015年 / 333卷

关键词：

Quick" baker's yeast; Maltase; Enzymatic properties; ALPHA-GLUCOSIDASE; SACCHAROMYCES-CEREVISIAE; LEAN DOUGH; MALTOSE; IDENTIFICATION;

D O I：

10.1007/978-3-662-46318-5_29

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The "quick" baker's yeasts are capable to rapidly metabolize maltose, thereby improving leavening ability. Given that maltase is the determining factor in maltose fermentation for "quick" baker's yeast, it is hence necessary to research the properties of the maltase independently for the "quick" baker's yeast. In this study, the heterogeneous expression of MAL62 encoding for maltase and purification of maltase were well completed. Furthermore, the enzymatic properties of maltase concentrated on the effects of substrate (maltose) and end product (glucose) were investigated as well as the physic-chemical properties and transglycosylation activity. The substrate maltose did not affect the activity of maltase, while the maltase activity was inhibited by the end product. This study provides guidance for the research of maltose metabolism for "quick" baker's yeast.

引用

页码：265 / 275

页数：11

共 50 条

[11] Removal Lead Ions from Solution by Nonliving Baker's Yeast
Dai, Qunwei
Dong, Faqin
Noonan, M. J.
Yand, Lijun
Wang, Zhongqi
[J]. PROGRESS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY, VOL II, PTS A AND B, 2009, : 1402 - 1407
[12] Synthesis of hydroquinone-α-glucoside by α-glucosidase from baker's yeast
Prodanovic, RM
Milosavic, NB
Sladic, DA
Velickovic, TC
Vujcic, Z
[J]. BIOTECHNOLOGY LETTERS, 2005, 27 (08) : 551 - 554
[13] Gene Expression Profiles of Baker's Yeast under Baking-associated Stresses
Nakamura, Toshihide
Ando, Akira
Shima, Jun
[J]. JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI, 2010, 57 (08): : 321 - 325
[14] Characterization of Maltase and Sucrase Inhibitory Constituents from Rhodiola crenulata
Li, Wen-Tai
Chuang, Yu-Hsuan
Hsieh, Jung-Feng
[J]. FOODS, 2019, 8 (11)
[15] Microbiological and Fermentative Properties of Baker's Yeast Starter Used in Breadmaking
Reale, A.
Di Renzo, T.
Succi, M.
Tremonte, P.
Coppola, R.
Sorrentino, E.
[J]. JOURNAL OF FOOD SCIENCE, 2013, 78 (08) : M1224 - M1231
[16] Preparation and studies on immobilized α-glucosidase from baker's yeast Saccharomyces cerevisiae
Ahmedi, Khaled S. O. H.
Milosaviz, Nenad B.
Popovic, Milica M.
Prodanovic, Radivoje M.
Knezevic, Zorica D.
Jankov, Ratko M.
[J]. JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 2007, 72 (12) : 1255 - 1263
[17] High-performance TiO2 from Baker's yeast
He, Wen
Cui, Jingjie
Yue, Yuanzheng
Zhang, Xudong
Xia, Xi
Liu, Hong
Lui, Suwen
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2011, 354 (01) : 109 - 115
[18] MAL62overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker's yeast in lean dough
Sun, Xi
Zhang, Jun
Fan, Zhi-Hua
Xiao, Ping
Li, Feng
Liu, Hai-Qing
Zhu, Wen-Bi
[J]. MICROBIAL CELL FACTORIES, 2020, 19 (01)
[19] A toolbox for systematic discovery of stable and transient protein interactors in baker's yeast
Fenech, Emma J.
Cohen, Nir
Kupervaser, Meital
Gazi, Zohar
Schuldiner, Maya
[J]. MOLECULAR SYSTEMS BIOLOGY, 2023, 19 (02)
[20] Functional Characterization of Extracellular Vesicles from Baker's Yeast Saccharomyces Cerevisiae as a Novel Vaccine Material for Immune Cell Maturation
Higuchi, Ayaka
Morishita, Masaki
Nagata, Ryoga
Maruoka, Kento
Katsumi, Hidemasa
Yamamoto, Akira
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 2023, 112 (02) : 525 - 534

← 1 2 3 4 5 →