Resuscitation of the rare boisphere contributes to pulses of ecosystem activity

被引:144
作者
Aanderud, Zachary T. [1 ]
Jones, Stuart E. [2 ]
Fierer, Noah [3 ,4 ,5 ]
Lennon, Jay T. [6 ]
机构
[1] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA
[2] Univ Notre Dame, Dept Biol Sci, South Bend, IN USA
[3] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA
[4] Univ Colorado, CIRES, Boulder, CO 80309 USA
[5] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA
[6] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA
基金
美国国家科学基金会; 美国食品与农业研究所;
关键词
CO2; pulses; dormancy; desiccation; dominance; stable isotope probing (SIP); soil rewetting; seed bank; rarity; PARTIAL LEAST-SQUARES; MICROBIAL DIVERSITY; RIBOSOMAL-RNA; CARBON-DIOXIDE; SEASONAL DYNAMICS; SOIL RESPIRATION; BACTERIA; MICROORGANISMS; COMMUNITIES; SURFACE;
D O I
10.3389/fmicb.2015.00024
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Dormancy is a life history trait that may have important implications for linking microbial communities to the functioning of natural and managed ecosystems. Rapid changes in environmental cues may resuscitate dormant bacteria and create pulses of ecosystem activity. In this study, we used heavy-water ((H2O)-O-18) stable isotope probing (SIP) to identify fast-growing bacteria that were associated with pulses of trace gasses (CO2, CH4, and N2O) from different ecosystems [agricultural site, grassland, deciduous forest, and coniferous forest (CF)] following a soil-rewetting event. Irrespective of ecosystem type, a large fraction (69-74%) of the bacteria that responded to rewetting were below detection limits in the dry soils. Based on the recovery of sequences, in just a few days, hundreds of rare taxa increased in abundance and in some cases became dominant members of the rewetted communities, especially bacteria belonging to the Sphingomonadaceae, Comamonadaceae, and Oxalobacteraceae. Resuscitation led to dynamic shifts in the rank abundance of taxa that caused previously rare bacteria to comprise nearly 60% of the sequences that were recovered in rewetted communities. This rapid turnover of the bacterial community corresponded with a 5-20-fold increase in the net production of CO2 and up to a 150% reduction in the net production of CH4 from rewetted soils. Results from our study demonstrate that the rare biosphere may account for a large and dynamic fraction of a community that is important for the maintenance of bacterial biodiversity. Moreover, our findings suggest that the resuscitation of rare taxa from seed banks contribute to ecosystem functioning.
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页数:11
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