Quantitative determination of trans-10-Hydroxy-2-Decenoic Acid (10-HDA) in Brazilian royal jelly and commercial products containing royal jelly

被引：12
作者：
Caparica-Santos, Celira
Marcucci, Maria Cristina
机构：
[1] Nat Lab Anal & Pesquisas Ltd, BR-13070350 Campinas, SP, Brazil
[2] Univ Bandeirante Sao Paulo, BR-02071013 Sao Paulo, Brazil
来源：
JOURNAL OF APICULTURAL RESEARCH | 2007年 / 46卷 / 03期
关键词：
Brazilian royal jelly; 10-HDA; HPLC; bee products; health; GC-MS;
D O I：
10.1080/00218839.2007.11101386
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
We report the analysis of Brazilian commercial bee products using high performance liquid chromatography, and a faster method, to detect trans-10-Hydroxy-2-Decenoic Acid or 10-Hydroxy-2-E-decenoic acid (10-HDA) in royal jelly. Determination was carried out using aqueous phosphoric acid 0.1 % (pH 2.5)/methanol (50/50) as the mobile phase, and a LiChrospher (R) RP-18 column with detection at 215 nm. A good linearity was shown between the concentration of 10-HDA and peak area in the concentration range of 5.0-50.0 mg/L (r 0.99, n = 5). The within-day and between-day relative standard deviation (RSD) were 1% and 3%, respectively.
引用
收藏
页码：149 / 153
页数：5
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