Antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. of human and animal origin isolated in Portugal

被引：26
作者：
Brandao, A. [2 ,3 ]
Almeida, T. [2 ,3 ]
Munoz-Atienza, E. [1 ]
Torres, C. [4 ]
Igrejas, G. [2 ,5 ]
Hernandez, P. E. [1 ]
Cintas, L. M. [1 ]
Poeta, P. [3 ,6 ]
Herranz, C. [1 ]
机构：
[1] Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, Grp Seguridad & Calidad Alimentos Bacterias Lact, E-28040 Madrid, Spain
[2] Univ Tras Os Montes & Alto Douro, Inst Biotechnol & Bioengn, Vila Real, Portugal
[3] Univ Tras Os Montes & Alto Douro, Dept Vet Sci, Vila Real, Portugal
[4] Univ La Rioja, Biochem & Mol Biol Area, Logrono, Spain
[5] Univ Tras Os Montes & Alto Douro, Ctr Genet & Biotechnol, Vila Real, Portugal
[6] Ctr Studies Anim & Vet Sci, Vila Real, Portugal
来源：
ARCHIVES OF MICROBIOLOGY | 2010年 / 192卷 / 11期
关键词：
Enterococcus spp; Antimicrobial activity; Bacteriocins; Enterocin structural genes; SEC-DEPENDENT BACTERIOCIN; DUCKS ANAS-PLATYRHYNCHOS; LACTIC-ACID BACTERIA; STREPTOCOCCUS-FAECALIS; FECAL ENTEROCOCCI; VIRULENCE FACTORS; WILD ANIMALS; FAECIUM L50; ANTIBIOTIC-RESISTANCE; ENTEROLYSIN-A;
D O I：
10.1007/s00203-010-0619-z
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The main objective of this study was to detect the antimicrobial activity and the presence of bacteriocin structural genes in 224 enterococcal isolates from fecal origin obtained from humans, pets, wild animals and birds. Direct antimicrobial activity against Listeria monocytogenes CECT4032 was detected in 102 (45.6%) of the tested isolates. From these, only 22 displayed bacteriocin activity against this indicator. The bacteriocinogenic strains contained one or more of the bacteriocin structural genes tested in this study, with those of enterocins P, A and L50 (L50A and L50B) being the most abundant. Our results show a high occurrence of the combination of different bacteriocin structural genes in the enterococcal isolates analyzed, indicating an elevated genetic potential of these strains to produce various bacteriocins.
引用
收藏
页码：927 / 936
页数：10
相关论文
共 43 条
  • [1] BAELE M, 2001, J APPL BACTERIOL, V91, P489
  • [2] Purification and characterization of two bacteriocins produced by lactic acid bacteria isolated from Mongolian airag
    Batdorj, B.
    Dalgalarrondo, M.
    Choiset, Y.
    Pedroche, J.
    Metro, F.
    Prevost, H.
    Chobert, J. -M.
    Haertle, T.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2006, 101 (04) : 837 - 848
  • [3] Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A
    Casaus, P
    Nilsen, T
    Cintas, LM
    Nes, IF
    Hernandez, PE
    Holo, H
    [J]. MICROBIOLOGY-SGM, 1997, 143 : 2287 - 2294
  • [4] Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum
    Cintas, LM
    Casaus, P
    Havarstein, L
    Hernandez, PE
    Nes, IF
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (11) : 4321 - 4330
  • [5] Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q
    Cintas, LM
    Casaus, P
    Herranz, C
    Håvarstein, LS
    Holo, H
    Hernández, PE
    Nes, IF
    [J]. JOURNAL OF BACTERIOLOGY, 2000, 182 (23) : 6806 - 6814
  • [6] Comparative antimicrobial activity of enterocin L50, pediocin PA-1, nisin A and lactocin S against spoilage and foodborne pathogenic bacteria
    Cintas, LM
    Casaus, P
    Fernandez, MF
    Hernandez, PE
    [J]. FOOD MICROBIOLOGY, 1998, 15 (03) : 289 - 298
  • [7] Enterocins L50A and L50B, two novel bacteriocins from Enterococcus faecium L50, are related to staphylococcal hemolysins
    Cintas, LM
    Casaus, P
    Holo, H
    Hernandez, PE
    Nes, IF
    Håvarstein, LS
    [J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (08) : 1988 - 1994
  • [8] ISOLATION AND CHARACTERIZATION OF PEDIOCIN L50, A NEW BACTERIOCIN FROM PEDIOCOCCUS-ACIDILACTICI WITH A BROAD INHIBITORY SPECTRUM
    CINTAS, LM
    RODRIGUEZ, JM
    FERNANDEZ, MF
    SLETTEN, K
    NES, IF
    HERNANDEZ, PE
    HOLO, H
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) : 2643 - 2648
  • [9] Cintas LM, 2001, FOOD SCI TECHNOL INT, V7, P281, DOI 10.1177/108201301772660538
  • [10] CITTI R, 2005, THESIS U COMPLUTENSE
12345