In this study, the influence of plasma treatment on the thermal comfort properties of cotton woven fabric has been investigated. The woven fabric sample was treated with three types of plasma, namely, air, argon, and oxygen plasma. The investigation was done with various parameters like treatment time, the distance between the fabric sample and electrode, and the frequency of the plasma process. It is observed that the air permeability of the fabric has a linear relationship with the distance of the sample and better air permeability achieved in air plasma. Also, the air permeability inversely related to time and frequency. The thermal resistance and water vapor permeability decreased with the distance and increased with the time and frequency. Similarly, the thermal resistance and water vapor permeability of cotton samples were enhanced in oxygen plasma treatment.