Mini-Tn7 vectors for studying post-transcriptional gene expression in Pseudomonas

被引：11

作者：

Liu, Yunhao ^{[1
,2
]}

Rainey, Paul B. ^{[2
,3
]}

Zhang, Xue-Xian ^{[1
]}

机构：

[1] Massey Univ Albany, Inst Nat & Math Sci, Auckland 0745, New Zealand

[2] Massey Univ Albany, NZ Inst Adv Study, Auckland 0745, New Zealand

[3] Max Planck Inst Evolutionary Biol, D-24306 Plon, Germany

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 107卷

关键词：

Gene regulation; LacZ; Noncoding RNAs; Translational fusion; Mini-Tn7; Pseudomonas; GRAM-NEGATIVE BACTERIA; FLUORESCENS SBW25; HISTIDINE UTILIZATION; ESCHERICHIA-COLI; SINGLE; SYSTEM; CONSTRUCTION; CHROMOSOMES; DIVERSITY; TRANSPORT;

D O I：

10.1016/j.mimet.2014.10.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the construction and validation of five mini-Tn7 vectors for analysis of post-transcriptional gene expression in Pseudomonas. Four vectors allow construction of translational fusions to beta-galactosidase (lacZ), while the fifth is designed for functional analysis of noncoding RNA genes. Translational fusions can be constructed without a functional promoter in the vector or from an inducible promoter of either P-tac or P-dctA. We show that promoterless fusions have value for determining levels of translation, whereas fusions to inducible promoters have utility in the analysis of mRNA-binding factors. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：182 / 185

页数：4

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