circRNA hsa_circ_0005909 Predicts Poor Prognosis and Promotes the Growth, Metastasis, and Drug Resistance of Non-Small-Cell Lung Cancer via the miRNA-338-3p/SOX4 Pathway

被引:18
作者
Song, He-Mei [1 ]
Meng, Dan [2 ]
Wang, Jin-Ping [3 ]
Zhang, Xiao-Yan [4 ]
机构
[1] Xuzhou Med Univ, Peoples Hosp Lianyungang 1, Dept Pharm, Affiliated Lianyungang Hosp, Lianyungang, Jiangsu, Peoples R China
[2] Qingdao Eighth Peoples Hosp, Dept Gen Surg, Qingdao, Shandong, Peoples R China
[3] Qingdao Eighth Peoples Hosp, Dept Gynaecol, Qingdao, Shandong, Peoples R China
[4] Weifang Peoples Hosp, Dept Pharm Intravenous Admixture, Weifang, Shandong, Peoples R China
关键词
CIRCULAR RNA; PROLIFERATION; SOX4; EXPRESSION; CERNA;
D O I
10.1155/2021/8388512
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background. Circular RNAs (circRNAs) are powerful factors in regulating various cancer behaviors. It has been manifested in previous researches that circular RNA hsa_circ_0005909 (circ_0005909) exhibits a regulatory function in osteosarcoma. However, there are no other studies on whether circ_0005909 displays potential functions on the progression of non-small-cell lung cancer (NSCLC). Methods. RT-PCR was applied to examine the expression of circ_0005909 in NSCLC. To study the specific behaviors of NSCLC cells after circ_0005909 knockdown, cell counting kit-8 (CCK-8) assays, colony formation assays, Transwell assays, and xenograft tumor model assays were conducted. Bioinformatics and luciferase reporter assays were employed to study the association among circ_0005909, miRNA-338-3p, and SOX4. Results. In this research, our group firstly showed that circ_0005909 expressions were distinctly increased in NSCLC specimens and cell lines. Clinical studies revealed that high circ_0005909 expressions were associated with poor prognosis of NSCLC patients. Functionally, knockdown of circ_0005909 was observed to suppress the proliferation, metastasis, and drug resistance of NSCLC cells. In the terms of mechanism, circ_0005909 could act as a sponge of miRNA-338-3p, and miRNA-338-3p could target SOX4. In addition, miRNA-338-3p inhibitors reversed the suppressor ability of circ_0005909 silence on NSCLC behaviors. Conclusions. circ_0005909 promoted the progression of NSCLC via the modulation of the miRNA-338-3p/SOX4 axis, which may be a therapeutic target for NSCLC.
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页数:15
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