MICOS and phospholipid transfer by Ups2-Mdm35 organize membrane lipid synthesis in mitochondria

被引:119
作者
Aaltonen, Mari J. [1 ,2 ]
Friedman, Jonathan R. [4 ]
Osman, Christof [1 ,2 ]
Salin, Benedicte [5 ]
Di Rago, Jean-Paul [5 ]
Nunnari, Jodi [4 ]
Langer, Thomas [1 ,2 ,3 ]
Tatsuta, Takashi [1 ,2 ]
机构
[1] Univ Cologne, Inst Genet, D-50931 Cologne, Germany
[2] Univ Cologne, Cologne Excellence Cluster Cellular Stress Respon, D-50931 Cologne, Germany
[3] Univ Cologne, Ctr Mol Med, D-50931 Cologne, Germany
[4] Univ Calif Davis, Dept Mol & Cellular Biol, Davis, CA 95616 USA
[5] Univ Bordeaux Segalen, Inst Biochim & Genet Cellulaires, CNRS, UMR5095, F-33077 Bordeaux, France
基金
欧洲研究理事会;
关键词
CONTACT SITES; INTRAMITOCHONDRIAL TRANSPORT; BIOSYNTHETIC PATHWAYS; PHOSPHATIDIC-ACID; PHOSPHATIDYLETHANOLAMINE; CARDIOLIPIN; YEAST; PROTEINS; CRISTAE; UPS1-MDM35;
D O I
10.1083/jcb.201602007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mitochondria exert critical functions in cellular lipid metabolism and promote the synthesis of major constituents of cellular membranes, such as phosphatidylethanolamine (PE) and phosphatidylcholine. Here, we demonstrate that the phosphatidylserine decarboxylase Psd 1, located in the inner mitochondrial membrane, promotes mitochondrial PE synthesis via two pathways. First, Ups2-Mdm35 complexes (SLMO2-TRIAP1 in humans) serve as phosphatidylserine (135)-specific lipid transfer proteins in the mitochondrial intermembrane space, allowing formation of PE by Psd1 in the inner membrane. Second, Psd1 decarboxylates PS in the outer membrane in trans, independently of PS transfer by Ups2-Mdm35. This latter pathway requires close apposition between both mitochondrial membranes and the mitochondrial contact site and cristae organizing system (MICOS). In MICOS-deficient cells, limiting PS transfer by Ups2-Mdm35 and reducing mitochondria! PE accumulation preserves mitochondrial respiration and cristae formation. These results link mitochondria! PE metabolism to MICOS, combining functions in protein and lipid homeostasis to preserve mitochondrial structure and function.
引用
收藏
页码:525 / 534
页数:10
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