Experimental Adaptation of the Yellow Fever Virus to the Mosquito Aedes albopictus and Potential risk of urban epidemics in Brazil, South America

被引：28

作者：

Amraoui, Fadila ^{[1
]}

Pain, Adrien ^{[2
]}

Piorkowski, Geraldine ^{[3
,4
]}

Vazeille, Marie ^{[1
]}

Couto-Lima, Dinair ^{[5
]}

de Lamballerie, Xavier ^{[3
,4
]}

Lourenco-de-Oliveira, Ricardo ^{[5
]}

Failloux, Anna-Bella ^{[1
]}

机构：

[1] Inst Pasteur, Dept Virol, Arboviruses & Insect Vectors Unit, Paris, France

[2] Inst Pasteur, Bioinformat & Biostat Hub C3BI, USR, IP CNRS 3756, Paris, France

[3] Aix Marseille Univ, IRD French Inst Res Dev, EHESP French Sch Publ Hlth, EPV UMR D Emergence Pathol Virales 190, Marseille, France

[4] Publ Hosp Marseille, AP HM, IHU Mediterranee Infect, Marseille, France

[5] Fiocruz MS, Inst Oswaldo Cruz, Lab Mosquitos Transmissores Hematozoarios, Rio De Janeiro, Brazil

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

PROTEIN NS1; AMAZONAS; MANAUS;

D O I：

10.1038/s41598-018-32198-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Despite the availability of an efficient vaccine, Yellow fever (YF), a viral disease transmitted by mosquitoes, is still a threat. In Brazil, the yellow fever virus (YFV) has been restricted to a jungle cycle for more than 70 years. However, YFV has recently invaded populated cities in the Southeast such as Rio de Janeiro where the opportunistic mosquito Aedes albopictus is well established. Using in vivo passages ofYFV in Ae. albopictus, we have selected viral strains presenting substitutions in NS1 gene. We did 10 passages ofYFV-74018 on two distinct Ae. albopictus populations: (i) Manaus collected from a YFV-endemic area in Amazonia and (ii) PNMNI from a YFV-free area in the state of Rio de Janeiro. Full viral genomes were deep sequenced at each passage. We obtained twoYFV strains presenting a non-synonymous substitution in the NS1 gene. Interestingly, they intervened at two different positions in NS1 gene according to the mosquito population: I2772T in Ae. albopictus Manaus and 53303N in Ae. albopictus PNMNI. Both substitutions reached fixation at the passage 10. Our data suggest thatYFV has the potential for adaption to Ae. albopictus thereby posing a threat to most cities in South America where this mosquito is present.

引用

页数：8