Applications of NMR to structure determination of RNAs large and small

被引：89

作者：

Barnwal, Ravi P. ^{[1
,2
]}

Yang, Fan ^{[1
]}

Varani, Gabriele ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[2] Panjab Univ, Dept Biophys, Chandigarh 160014, India

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2017年 / 628卷

关键词：

RNA; Isotope labeling; Structure; NMR spectroscopy; RESIDUAL DIPOLAR COUPLINGS; SEQUENTIAL BACKBONE ASSIGNMENT; THROUGH-BOND CORRELATION; HIGH-RESOLUTION NMR; MACROMOLECULAR STRUCTURE DETERMINATION; PERFORMANCE LIQUID-CHROMATOGRAPHY; TRIPLE-RESONANCE EXPERIMENTS; NUCLEAR-MAGNETIC-RESONANCE; ADENINE H2/H8 RESONANCES; MURINE LEUKEMIA-VIRUS;

D O I：

10.1016/j.abb.2017.06.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool to investigate the structure and dynamics of RNA, because many biologically important RNAs have conformationally flexible structures, which makes them difficult to crystallize. Functional, independently folded RNA domains, range in size between simple stem-loops of as few as 10-20 nucleotides, to 50-70 nucleotides, the size of tRNA and many small ribozymes, to a few hundred nucleotides, the size of more complex RNA enzymes and of the functional domains of non-coding transcripts. In this review, we discuss new methods for sample preparation, assignment strategies and structure determination for independently folded RNA domains of up to 100 kDa in molecular weight. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：42 / 56

页数：15

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