Laccase from a non-melanogenic, alkalotolerant γ-proteobacterium JB isolated from industrial wastewater drained soil

被引：53

作者：

Bains, J

Capalash, N

Sharma, P ^{[1
]}

机构：

[1] Panjab Univ, Dept Microbiol, Chandigarh 160014, India

[2] Panjab Univ, Dept Biotechnol, Chandigarh 160014, India

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 14期

关键词：

alkalotolerance; extracellular; gamma-proteobacterium; laccase;

D O I：

10.1023/A:1024569722413

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Gram-negative, alkalotolerant bacterium, isolated from the soil continually drained with industrial wastewater and identified as gamma-proteobacteriumby partial 16S rRNA sequence analysis, produced a polyphenol oxidase, which showed laccase but not tyrosinase activity. The organism grew well from pH 6 to 10 and produced laccase maximally at pH 10. The enzyme was stable from pH 3 to 10.6 for at least 24 h and was optimally active at 55 degreesC and pH 6.5 in a 5 min assay.

引用

页码：1155 / 1159

页数：5

共 14 条

[1] Laccases are widespread in bacteria [J].