Laccase from a non-melanogenic, alkalotolerant γ-proteobacterium JB isolated from industrial wastewater drained soil

被引：52
作者：
Bains, J
Capalash, N
Sharma, P [1 ]
机构：
[1] Panjab Univ, Dept Microbiol, Chandigarh 160014, India
[2] Panjab Univ, Dept Biotechnol, Chandigarh 160014, India
来源：
BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 14期
关键词：
alkalotolerance; extracellular; gamma-proteobacterium; laccase;
D O I：
10.1023/A:1024569722413
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A Gram-negative, alkalotolerant bacterium, isolated from the soil continually drained with industrial wastewater and identified as gamma-proteobacteriumby partial 16S rRNA sequence analysis, produced a polyphenol oxidase, which showed laccase but not tyrosinase activity. The organism grew well from pH 6 to 10 and produced laccase maximally at pH 10. The enzyme was stable from pH 3 to 10.6 for at least 24 h and was optimally active at 55 degreesC and pH 6.5 in a 5 min assay.
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页码：1155 / 1159
页数：5
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