The splicing factor SR45 affects the RNA-directed DNA methylation pathway in Arabidopsis

被引：54

作者：

Ausin, Israel ^{[1
,2
]}

Greenberg, Maxim V. C. ^{[1
]}

Li, Carey Fei ^{[1
,3
]}

Jacobsen, Steven E. ^{[1
,4
]}

机构：

[1] Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain

[2] Campus Univ Autonoma Madrid, Dept Plant Mol Genet, Madrid, Spain

[3] Univ Calif Irvine, Dept Neurol, Inst Immunol, Irvine, CA 92717 USA

[4] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90024 USA

来源：

EPIGENETICS | 2012年 / 7卷 / 01期

基金：

美国国家卫生研究院;

关键词：

DNA methylation; Arabidopsis; de novo; genetic screen; siRNA; DRM; METHYLTRANSFERASES; VERNALIZATION; PLANTS;

D O I：

10.4161/epi.7.1.18782

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosine DNA methylation is an epigenetic mark frequently associated with silencing of genes and transposons. In Arabidopsis, the establishment of cytosine DNA methylation is performed by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2). DRM2 is guided to target sequences by small interfering RNAs (siRNAs) in a pathway termed RNA-directed DNA methylation (RdDM). We performed a screen for mutants that affect the establishment of DNA methylation by investigating genes that contain predicted RNA-interacting domains. After transforming FWA into 429 T-DNA insertion lines, we assayed for mutants that exhibited a late-flowering phenotype due to hypomethylated, thus ectopically expressed, copies of FWA. A T-DNA insertion line within the coding region of the spliceosome gene SR45 (sr45-1) flowered late after FWA transformation. Additionally, sr45-1 mutants display defects in the maintenance of DNA methylation. DNA methylation establishment and maintenance defects present in sr45-1 mutants are enhanced in dcl3-1 mutant background, suggesting a synergistic cooperation between SR45 and DICER-LIKE3 (DCL3) in the RdDM pathway.

引用

页码：29 / 33

页数：5

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