共 19 条
The splicing factor SR45 affects the RNA-directed DNA methylation pathway in Arabidopsis
被引:54
作者:

Ausin, Israel
论文数: 0 引用数: 0
h-index: 0
机构:
Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain
Campus Univ Autonoma Madrid, Dept Plant Mol Genet, Madrid, Spain Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain

Greenberg, Maxim V. C.
论文数: 0 引用数: 0
h-index: 0
机构:
Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain

Li, Carey Fei
论文数: 0 引用数: 0
h-index: 0
机构:
Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain
Univ Calif Irvine, Dept Neurol, Inst Immunol, Irvine, CA 92717 USA Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain

Jacobsen, Steven E.
论文数: 0 引用数: 0
h-index: 0
机构:
Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain
Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90024 USA Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain
机构:
[1] Campus Univ Autonoma Madrid, Dept Mol Cell & Dev Biol, Madrid, Spain
[2] Campus Univ Autonoma Madrid, Dept Plant Mol Genet, Madrid, Spain
[3] Univ Calif Irvine, Dept Neurol, Inst Immunol, Irvine, CA 92717 USA
[4] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90024 USA
来源:
基金:
美国国家卫生研究院;
关键词:
DNA methylation;
Arabidopsis;
de novo;
genetic screen;
siRNA;
DRM;
METHYLTRANSFERASES;
VERNALIZATION;
PLANTS;
D O I:
10.4161/epi.7.1.18782
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Cytosine DNA methylation is an epigenetic mark frequently associated with silencing of genes and transposons. In Arabidopsis, the establishment of cytosine DNA methylation is performed by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2). DRM2 is guided to target sequences by small interfering RNAs (siRNAs) in a pathway termed RNA-directed DNA methylation (RdDM). We performed a screen for mutants that affect the establishment of DNA methylation by investigating genes that contain predicted RNA-interacting domains. After transforming FWA into 429 T-DNA insertion lines, we assayed for mutants that exhibited a late-flowering phenotype due to hypomethylated, thus ectopically expressed, copies of FWA. A T-DNA insertion line within the coding region of the spliceosome gene SR45 (sr45-1) flowered late after FWA transformation. Additionally, sr45-1 mutants display defects in the maintenance of DNA methylation. DNA methylation establishment and maintenance defects present in sr45-1 mutants are enhanced in dcl3-1 mutant background, suggesting a synergistic cooperation between SR45 and DICER-LIKE3 (DCL3) in the RdDM pathway.
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页码:29 / 33
页数:5
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