A photostable monomeric superfolder green fluorescent protein

被引：26

作者：

Valbuena, Fernando M. ^{[1
]}

Fitzgerald, Ivy ^{[2
]}

Strack, Rita L. ^{[1
]}

Andruska, Neal ^{[1
]}

Smith, Luke ^{[1
]}

Glick, Benjamin S. ^{[1
]}

机构：

[1] Univ Chicago, Dept Mol Genet & Cell Biol, 920 East 58th St, Chicago, IL 60637 USA

[2] Univ Chicago, Grad Program Biophys Sci, Chicago, IL 60637 USA

来源：

TRAFFIC | 2020年 / 21卷 / 08期

关键词：

cytotoxicity; fluorescent protein; GFP; mCherry; monomeric; mScarlet; photobleaching; superfolder; ENDOPLASMIC-RETICULUM; PICHIA-PASTORIS; YEAST; INHERITANCE; HOMOLOG; VARIANT; RED; GFP;

D O I：

10.1111/tra.12737

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The green fluorescent protein (GFP) fromAequorea victoriahas been engineered extensively in the past to generate variants suitable for protein tagging. Early efforts produced the enhanced variant EGFP and its monomeric derivative mEGFP, which have useful photophysical properties, as well as superfolder GFP, which folds efficiently under adverse conditions. We previously generated msGFP, a monomeric superfolder derivative of EGFP. Unfortunately, compared to EGFP, msGFP and other superfolder GFP variants show faster photobleaching. We now describe msGFP2, which retains monomeric superfolder properties while being as photostable as EGFP. msGFP2 contains modified N- and C-terminal peptides that are expected to reduce nonspecific interactions. Compared to EGFP and mEGFP, msGFP2 is less prone to disturbing the functions of certain partner proteins. For general-purpose protein tagging, msGFP2 may be the best available derivative ofA. victoriaGFP.

引用

页码：534 / 544

页数：11

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