The HIV-1 repeated sequence R as a robust hot-spot for copy-choice recombination

被引:50
作者
Moumen, A
Polomack, L
Roques, B
Buc, H
Negroni, M
机构
[1] Inst Pasteur, CNRS, Unite Regulat Enzymat Activ Cellulaires, FRE 2364,Dept Biol Mol, F-75724 Paris 15, France
[2] Inst Pasteur, CNRS, URA 1960, F-75724 Paris 15, France
[3] CNRS, Dept Pharmacochim Mol & Struct, INSERM, U266,UMR 8600, F-75270 Paris 06, France
关键词
D O I
10.1093/nar/29.18.3814
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Template switching during reverse transcription is crucial for retroviral replication. While strand transfer on the terminal repeated sequence R is essential to achieve reverse transcription, template switching from internal regions of the genome (copy choice) leads to genetic recombination. We have developed an experimental system to study copy-choice recombination in vitro along the HIV-1 genome. We identify here several genomic regions, including the R sequence, where copy choice occurred at high rates. The frequency of copy choice occurring in a given region of template was strongly influenced by the surrounding sequences, an observation that suggests a pivotal role of the folding of template RNA in the process. The sequence R, instead, constituted an exception to this rule since it was a strong hot-spot for copy choice in the different sequence contexts tested. We suggest therefore that the structure of this region has been optimised during viral evolution to ensure efficient template switching independently from the sequences that might surround it.
引用
收藏
页码:3814 / 3821
页数:8
相关论文
共 44 条
[1]   CIS elements and trans-acting factors required for minus strand DNA transfer during reverse transcription of the genomic RNA of murine leukemia virus [J].
Allain, B ;
Rascle, JB ;
de Rocquigny, H ;
Roques, B ;
Darlix, JL .
JOURNAL OF MOLECULAR BIOLOGY, 1998, 277 (02) :225-235
[2]   Structural features in the HIV-1 repeat region facilitate strand transfer during reverse transcription [J].
Berkhout, B ;
Vastenhouw, NL ;
Klasens, BIF ;
Huthoff, H .
RNA, 2001, 7 (08) :1097-1114
[3]   Utilization of nonviral sequences for minus-strand DNA transfer and gene reconstitution during retroviral replication [J].
Cheslock, SR ;
Anderson, JA ;
Hwang, CK ;
Pathak, VK ;
Hu, WS .
JOURNAL OF VIROLOGY, 2000, 74 (20) :9571-9579
[4]   FIRST GLIMPSES AT STRUCTURE-FUNCTION-RELATIONSHIPS OF THE NUCLEOCAPSID PROTEIN OF RETROVIRUSES [J].
DARLIX, JL ;
LAPADATTAPOLSKY, M ;
DEROCQUIGNY, H ;
ROQUES, BP .
JOURNAL OF MOLECULAR BIOLOGY, 1995, 254 (04) :523-537
[5]   VIRAL-RNA ANNEALING ACTIVITIES OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NUCLEOCAPSID PROTEIN REQUIRE ONLY PEPTIDE DOMAINS OUTSIDE THE ZINC FINGERS [J].
DEROCQUIGNY, H ;
GABUS, C ;
VINCENT, A ;
FOURNIEZALUSKI, MC ;
ROQUES, B ;
DARLIX, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6472-6476
[6]   REQUIREMENTS FOR STRAND TRANSFER BETWEEN INTERNAL REGIONS OF HETEROPOLYMER TEMPLATES BY HUMAN-IMMUNODEFICIENCY-VIRUS REVERSE-TRANSCRIPTASE [J].
DESTEFANO, JJ ;
MALLABER, LM ;
RODRIGUEZRODRIGUEZ, L ;
FAY, PJ ;
BAMBARA, RA .
JOURNAL OF VIROLOGY, 1992, 66 (11) :6370-6378
[7]   Evidence of interactions between the nucleocapsid protein NCp7 and the reverse transcriptase of HIV-1 [J].
Druillennec, S ;
Caneparo, A ;
de Rocquigny, H ;
Roques, BP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (16) :11283-11288
[8]   DETAILED MODEL OF REVERSE TRANSCRIPTION AND TESTS OF CRUCIAL ASPECTS [J].
GILBOA, E ;
MITRA, SW ;
GOFF, S ;
BALTIMORE, D .
CELL, 1979, 18 (01) :93-100
[9]   Human immunodeficiency virus type 1 nucleocapsid protein promotes efficient strand transfer and specific viral DNA synthesis by inhibiting TAR-dependent self-priming from minus-strand strong-stop DNA [J].
Guo, JH ;
Henderson, LE ;
Bess, J ;
Kane, B ;
Levin, JG .
JOURNAL OF VIROLOGY, 1997, 71 (07) :5178-5188
[10]   The effect of mutations in the HIV-1 nucleocapsid protein on strand transfer in cell-free reverse transcription reactions [J].
Hsu, Mayla ;
Rong, Liwei ;
de Rocquigny, Hugues ;
Roques, Bernard P. ;
Wainberg, Mark A. .
NUCLEIC ACIDS RESEARCH, 2000, 28 (08) :1724-1729