Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay

被引:94
作者
Cho, YJ
Lee, DH
Kim, DO
Min, WK
Bong, KT
Lee, GG
Seo, JH [1 ]
机构
[1] Seoul Natl Univ, Sch Agr Biotechnol, Seoul 151742, South Korea
[2] Seoul Natl Univ, Ctr Agr Biomat, Seoul 151742, South Korea
[3] Samsung Everland Inc, Food Serv, Food Res & Dev Ctr, Yongin 449912, South Korea
[4] Samsung Everland Inc, Distribut Div, Yongin 449912, South Korea
关键词
ochratoxin A; monoclonal antibody; enzyme-linked immunosorbent assay (ELISA); colloidal gold; immunochromatographic assay;
D O I
10.1021/jf051681q
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
A monoclonal antibody (Mab) against ochratoxin A (OTA) was produced from the hybridoma cell line C7G25, which was established by the fusion of Sp2/0-Ag14 myeloma cells with spleen cells isolated from a BALB/c mouse immunized with the OTA-bovine serum albumin conjugate. This Mab belongs to the IgG(2a) heavy-chain subclass with a kappa-type light chain. The level of 50% inhibition concentration was 1.20 ng/mL in a competitive direct enzyme-linked immunosorbent assay (cdELISA), and the detection limit was 0.12 ng/mL. This antibody is specific for OTA but also shows cross-reactivity with ochratoxin B (31.7%) in a cdELISA. On the basis of the sandwich format using the produced Mab against OTA, a rapid immunochromatographic assay was developed to efficiently detect OTA. This method was able to detect up to 500 ng/mL of OTA in <10 min.
引用
收藏
页码:8447 / 8451
页数:5
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