Analysis of microRNA-size, small RNAs in Streptococcus mutans by deep sequencing

被引：67

作者：

Lee, Heon-Jin ^{[1
]}

Hong, Su-Hyung ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Dept Oral Microbiol, Sch Dent, Taegu 700412, South Korea

来源：

FEMS MICROBIOLOGY LETTERS | 2012年 / 326卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

miRNA; small RNA; sRNA; Streptococcus mutans; deep sequencing; REGULATORY RNAS; BACTERIA; IDENTIFICATION; MECHANISM;

D O I：

10.1111/j.1574-6968.2011.02441.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MicroRNAs (miRNAs) are important modulators of gene expression in eukaryotic cells. However RNAs of the same size in bacteria have not been specifically discussed previously. Here, we provide a library of miRNA-size RNAs (msRNAs), which were registered by deep sequencing in Streptococcus mutans. Bioinformatic analysis of the whole set revealed more than 900 individual msRNA species. The cellular content of selected msRNAs was verified by quantitative RT-PCR and Northern blotting. The high abundance and discrete size of the subset of registered msRNAs suggest their functional significance, although the precise biological role of the RNA species revealed in S. mutans which is one of the principle causative agents of dental caries, has to be elucidated.

引用

页码：131 / 136

页数：6

共 23 条

[1] Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen [J].