The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells

被引：31
作者：
Ahn, Jae Hwa [1 ]
Kim, In-Ryoung [2 ]
Kim, Yeon [3 ]
Kim, Dong-Hyun [4 ]
Park, Soo-Byung [1 ]
Park, Bong-Soo [2 ]
Bae, Moon-Kyoung [3 ]
Kim, Yong-I [1 ,5 ]
机构：
[1] Pusan Natl Univ, Dent Res Inst, Dept Orthodont, Yangsan 50612, South Korea
[2] Pusan Natl Univ, Sch Dent, Dept Oral Anant, Yangsan 50612, South Korea
[3] Pusan Natl Univ, Sch Dent, Dept Oral Physiol, Yangsan 50612, South Korea
[4] DAEWON Mat Co Ltd, R&D Ctr, 365,Sinseon Ro, Busan 48547, South Korea
[5] Pusan Natl Univ, Dent & Life Sci Inst, Yangsan 50612, South Korea
来源：
NANOMATERIALS | 2020年 / 10卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
bioactive glass; graphene oxide; hDPSC; odontogenic differentiation; mineralization; GRAPHENE OXIDE; IN-VITRO; MATRIX PROTEIN-1; BIOMEDICAL APPLICATIONS; MECHANICAL-PROPERTIES; BONE; SCAFFOLDS; ROLES; SIALOPHOSPHOPROTEIN; BIOMINERALIZATION;
D O I：
10.3390/nano10040620
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The purpose of this study was to investigate the effects of mesoporous bioactive glass nanoparticle (MBN)/graphene oxide (GO) composites on the mineralization ability and differentiation potential of human dental pulp stem cells (hDPSCs). MBN/GO composites were synthesized using the sol-gel method and colloidal processing to enhance the bioactivity and mechanical properties of MBN. Characterization using FESEM, XRD, FTIR, and Raman spectrometry showed that the composites were successfully synthesized. hDPSCs were then cultured directly on the MBN/GO (40:1 and 20:1) composites in vitro. MBN/GO promoted the proliferation and alkaline phosphatase (ALP) activity of hDPSCs. In addition, qRT-PCR showed that MBN/GO regulated the mRNA levels of odontogenic markers (dentin sialophosphoprotein (DSPP), dentine matrix protein 1 (DMP-1), ALP, matrix extracellular phosphoglycoprotein (MEPE), bone morphogenetic protein 2 (BMP-2), and runt-related transcription factor 2 (RUNX-2)). The mRNA levels of DSPP and DMP-1, two odontogenesis-specific markers, were considerably upregulated in hDPSCs in response to growth on the MBN/GO composites. Western blot analysis revealed similar results. Alizarin red S staining was subsequently performed to further investigate MBN/GO-induced mineralization of hDPSCs. It was revealed that MBN/GO composites promote odontogenic differentiation via the Wnt/beta-catenin signaling pathway. Collectively, the results of the present study suggest that MBN/GO composites may promote the differentiation of hDPSCs into odontoblast-like cells, and potentially induce dentin formation.
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页数：18
相关论文
共 58 条
[1]   Wnt/β-catenin signaling in bone marrow niche [J].
Ahmadzadeh, Ahmad ;
Norozi, Fatemeh ;
Shahrabi, Saeid ;
Shahjahani, Mohammad ;
Saki, Najmaldin .
CELL AND TISSUE RESEARCH, 2016, 363 (02) :321-335
[2]   MAPPING OF THE HUMAN DENTIN MATRIX ACIDIC PHOSPHOPROTEIN GENE (DMP1) TO THE DENTINOGENESIS IMPERFECTA TYPE-II CRITICAL REGION AT CHROMOSOME 4Q21 [J].
APLIN, HM ;
HIRST, KL ;
CROSBY, AH ;
DIXON, MJ .
GENOMICS, 1995, 30 (02) :347-349
[3]   Mechanical properties and biomedical applications of a nanotube hydroxyapatite-reduced graphene oxide composite [J].
Baradaran, S. ;
Moghaddam, E. ;
Basirun, W. J. ;
Mehrali, M. ;
Sookhakian, M. ;
Hamdi, M. ;
Moghaddam, M. R. Nakhaei ;
Alias, Y. .
CARBON, 2014, 69 :32-45
[4]   Polymer/bioactive glass nanocomposites for biomedical applications: A review [J].
Boccaccini, Aldo R. ;
Erol, Melek ;
Stark, Wendelin J. ;
Mohn, Dirk ;
Hong, Zhongkui ;
Mano, Joao F. .
COMPOSITES SCIENCE AND TECHNOLOGY, 2010, 70 (13) :1764-1776
[5]   Dentin sialoprotein (DSP) has limited effects on in vitro apatite formation and growth [J].
Boskey, A ;
Spevak, L ;
Tan, M ;
Doty, SB ;
Butler, WT .
CALCIFIED TISSUE INTERNATIONAL, 2000, 67 (06) :472-478
[6]  
Boskey AL, 1998, J CELL BIOCHEM, P83, DOI 10.1002/(SICI)1097-4644(1998)72:30/31+<83::AID-JCB12>3.0.CO
[7]  
2-F
[8]   In vitro toxicity evaluation of graphene oxide on A549 cells [J].
Chang, Yanli ;
Yang, Sheng-Tao ;
Liu, Jia-Hui ;
Dong, Erya ;
Wang, Yanwen ;
Cao, Aoneng ;
Liu, Yuanfang ;
Wang, Haifang .
TOXICOLOGY LETTERS, 2011, 200 (03) :201-210
[9]   A graphene-based platform for induced pluripotent stem cells culture and differentiation [J].
Chen, G. -Y. ;
Pang, D. W. -P. ;
Hwang, S. -M. ;
Tuan, H. -Y. ;
Hu, Y. -C. .
BIOMATERIALS, 2012, 33 (02) :418-427
[10]   Preparation of graphene by the rapid and mild thermal reduction of graphene oxide induced by microwaves [J].
Chen, Wufeng ;
Yan, Lifeng ;
Bangal, Prakriti R. .
CARBON, 2010, 48 (04) :1146-1152
123456