Cloning and characterization of a novel UDP-glycosyltransferase gene induced by DON from wheat

被引:22
作者
Ma Xin [1 ]
Du Xu-ye [1 ]
Liu Guo-juan [1 ]
Yang Zai-dong [1 ]
Hou Wen-qian [1 ]
Wang Hong-wei [1 ]
Feng De-shun [1 ]
Li An-fei [1 ]
Kong Ling-rang [1 ]
机构
[1] Shandong Agr Univ, Coll Agron, Shandong Key Lab Crop Biol, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
基金
国家教育部博士点专项基金资助; 中国国家自然科学基金;
关键词
UDP-glycosyltransferase; Fusarium graminearum; deoxynivalenol; wheat; FUSARIUM HEAD BLIGHT; MYCOTOXIN DEOXYNIVALENOL; HETEROLOGOUS EXPRESSION; SALICYLIC-ACID; SPRING WHEAT; CROSS-TALK; RESISTANCE; GLUCOSYLTRANSFERASE; BARLEY; QTLS;
D O I
10.1016/S2095-3119(14)60857-1
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a destructive disease of wheat throughout the world. However, the mechanisms of host resistance to FHB are still largely unclear. Deoxynivalenol (DON) produced by Fusarium graminearum which enhances the pathogen to spread could be converted into inactive form D3G by UDP-glycosyltransferases (UGTs). A DON responsive UGT gene, designated as TaUGT4, was first cloned from wheat in this study. The putative open reading frame (ORF) of TaUGT4 was 1 386 bp, encoding 461 amino acids protein. TaUGT4 was placed on chromosome 2D using a set of nulli-tetrasomic lines of wheat cultivar Chinese Spring (CS). When fused with eGFP at C terminal, TaUGT4 was shown to localize in cytoplasm of the transformed tobacco cells. The transcriptional analysis revealed that TaUGT4 was strongly induced by Fusarium graminearum or DON in both of FHB-resistant cultivar Sumai 3 and susceptible cultivar Kenong 199, especially in Sumai 3 under DON treatment. Similar increase of TaUGT4 expression was observed in Sumai 3 and Kenong 199 in response to salicylic acid (SA) treatment. But interestingly, the transcripts level of TaUGT4 in Sumai 3 showed significantly higher than that in Kenong 199 after treated with methyl jasmonate (MeJA). According to the expression patterns, TaUGT4 might lead to different effects between FHB-resistant genotype and susceptible genotype in the process against Fusarium graminearum inoculation. It had also been discussed in this paper that JA signaling pathway might play a significant role in the resistance against Fusarium graminearum compared to SA signaling pathway.
引用
收藏
页码:830 / 838
页数:9
相关论文
共 50 条
[11]   Efficient Biosynthesis of Gastrodin by UDP-Glycosyltransferase from Rauvolfia serpentina [J].
Ge, Lin ;
Xia, Yu ;
Xu, Wenxin ;
Jia, Ruobing ;
Zhang, Tingting .
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2025, 35
[12]   Characterization of the UDP-glycosyltransferase UGT33D1 in silkworm Bombyx mori [J].
Zhu, Feifei ;
Han, Jinying ;
Hong, Jingdie ;
Cai, Fuchuan ;
Tang, Qi ;
Yu, Qian ;
Ma, Shangshang ;
Liu, Xiaoyong ;
Huo, Shuhao ;
Chen, Keping .
INSECT MOLECULAR BIOLOGY, 2024, 33 (06) :697-707
[13]   Enzymatic biosynthesis of novel neobavaisoflavone glucosides via Bacillus UDP-glycosyltransferase [J].
MA Tao ;
DAI YiQun ;
LI Nan ;
HUO Qiang ;
LI HongMei ;
ZHANG YuXin ;
PIAO ZhengHao ;
WU ChengZhu .
ChineseJournalofNaturalMedicines, 2017, 15 (04) :281-287
[14]   Molecular Identification and Characterization of UDP-glycosyltransferase (UGT) Multigene Family in Pomegranate [J].
Zhao, Xueqing ;
Feng, Yingyi ;
Ke, Ding ;
Teng, Yingfen ;
Chen, Ying ;
Langjia, Renzeng .
HORTICULTURAE, 2023, 9 (05)
[15]   Enzymatic biosynthesis of novel bavachin glucosides via Bacillus UDP-glycosyltransferase [J].
Dai, Yiqun ;
Zhang, Songsong ;
Liu, Da-Chuan ;
Li, Hong-Mei ;
Ma, Tao ;
Huo, Qiang ;
Wu, Cheng-Zhu .
PHYTOCHEMISTRY LETTERS, 2018, 23 :9-14
[16]   Characterization and protein engineering of a novel UDP-glycosyltransferase involved in pseudoginsenoside Rt5 biosynthesis from Panax japonicus [J].
Li, Pengfei ;
Huang, Chaokang ;
Niu, Tengfei ;
Yang, Xiaolin ;
Guan, Huida ;
Ding, Lili ;
Yang, Li ;
Wang, Zhengtao ;
Pu, Zhongji ;
Wang, Rufeng .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2024, 277
[17]   UDP-glycosyltransferase gene family expansion and functional perspectives in five tenebrionid beetles [J].
Li, Xun ;
Yang, Yanlin ;
Wei, Shujun ;
Zhu, Jiaying .
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, 2024, 27 (02)
[18]   Similarities in Structure and Function of UDP-Glycosyltransferase Homologs from Human and Plants [J].
Lethe, Mary Caroline L. ;
Paris, Vincent ;
Wang, Xiaoqiang ;
Chan, Clement T. Y. .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (05)
[19]   Genome-wide characterization of the UDP-glycosyltransferase gene family reveals their potential roles in leaf senescence in cotton [J].
Chen, Yizhen ;
Fu, Mingchuan ;
Li, Hao ;
Wang, Liguo ;
Liu, Renzhong ;
Liu, Zhanji .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, 222 :2648-2660
[20]   The UDP-Glycosyltransferase Gene Family in Achelura yunnanensis (Lepidoptera: Zygaenidae): Identification, Phylogeny, and Diverse Expression Patterns [J].
Xiao, Hai-Yan ;
Chen, Dan-Lu ;
Lu, Ting-Ting ;
Yao, Yu-Juan ;
Liu, Nai-Yong .
DIVERSITY-BASEL, 2022, 14 (05)