Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology

We present a detailed protocol for gene editing in adipocytes using the CRISPR-Cas technology. This protocol describes sgRNA design, preparation of lenti-CRISPR-sgRNA vectors, functional validation of sgRNAs, preparation of lentivi-ruses, and lentiviruses transduction in adipocytes. Moreover, an optimized method of gene editing using the lentiCRISPRv2 vector expressing two sgRNAs targeting two different genes has also been described.For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).