Mkit: A cell migration assay based on microfluidic device and smartphone

被引：25

作者：

Yang, Ke ^{[1
,2
]}

Wu, Jiandong ^{[2
]}

Peretz-Soroka, Hagit ^{[2
]}

Zhu, Ling ^{[1
]}

Li, Zhigang ^{[1
]}

Sang, Yaoshuo ^{[1
]}

Hipolito, Jolly ^{[2
]}

Zhang, Michael ^{[3
]}

Santos, Susy ^{[4
,5
]}

Hillier, Craig ^{[3
]}

de Faria, Ricardo Lobato ^{[3
]}

Liu, Yong ^{[1
]}

Lin, Francis ^{[2
,6
,7
,8
]}

机构：

[1] Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei, Anhui, Peoples R China

[2] Univ Manitoba, Dept Phys & Astron, Winnipeg, MB, Canada

[3] Seven Oaks Gen Hosp, Winnipeg, MB, Canada

[4] Victoria Gen Hosp, Winnipeg, MB, Canada

[5] River Hts Ft Garry Community Areas, Winnipeg, MB, Canada

[6] Univ Manitoba, Dept Biosyst Engn, Winnipeg, MB, Canada

[7] Univ Manitoba, Dept Immunol, Winnipeg, MB, Canada

[8] Univ Manitoba, Dept Biol Sci, Winnipeg, MB, Canada

来源：

BIOSENSORS & BIOELECTRONICS | 2018年 / 99卷

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

Microfluidic device; Smartphone; Cell functional assay; Cell migration; Chemotaxis; POINT-OF-CARE; NEUTROPHIL CHEMOTAXIS; BIOMEDICAL-RESEARCH; MINI-MICROSCOPE; MOBILE PHONE; BLOOD; PLATFORM; SYSTEM; INFLAMMATION; TUMOR;

D O I：

10.1016/j.bios.2017.07.064

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS2-based cell functional assay for testing cell migration (the Midt). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the Mkit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the Midt with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the Midt. In addition to research applications, we demonstrated the effective use of the Mkit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed Mkit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.

引用

页码：259 / 267

页数：9

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