Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy

被引：30

作者：

Xing, Feiyue ^{[1
]}

Wang, Jiongkun ^{[1
]}

Hu, Mingqian ^{[2
]}

Yu, Yu ^{[3
,4
]}

Chen, Guoliang ^{[1
]}

Liu, Jing ^{[5
]}

机构：

[1] Jinan Univ, Inst Tissue Transplantat & Immunol, Guangzhou 510632, Guangdong, Peoples R China

[2] Jinan Univ, Dept Chem, Guangzhou 510632, Guangdong, Peoples R China

[3] H Lee Moffitt Canc Ctr & Res Inst, Dept Immunol, Tampa, FL 33612 USA

[4] H Lee Moffitt Canc Ctr & Res Inst, Dept Blood & Marrow Transplantat, Tampa, FL 33612 USA

[5] Jinan Univ, Dept Stomatol, Guangzhou 510632, Guangdong, Peoples R China

来源：

NANOSCALE RESEARCH LETTERS | 2011年 / 6卷

基金：

中国国家自然科学基金;

关键词：

dendritic cell; nanostructure; adhesion force; comparison; GM-CSF; MATURATION; SURVIVAL; AFM; DIFFERENTIATION; KINASE; IL-4; SEM;

D O I：

10.1186/1556-276X-6-455

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A comparative study of immature and mature bone marrow-derived dendritic cells (BMDCs) was first performed through an atomic force microscope (AFM) to clarify differences of their nanostructure and adhesion force. AFM images revealed that the immature BMDCs treated by granulocyte macrophage-colony stimulating factor plus IL-4 mainly appeared round with smooth surface, whereas the mature BMDCs induced by lipopolysaccharide displayed an irregular shape with numerous pseudopodia or lamellapodia and ruffles on the cell membrane besides becoming larger, flatter, and longer. AFM quantitative analysis further showed that the surface roughness of the mature BMDCs greatly increased and that the adhesion force of them was fourfold more than that of the immature BMDCs. The nano-features of the mature BMDCs were supported by a high level of IL-12 produced from the mature BMDCs and high expression of MHC-II on the surface of them. These findings provide a new insight into the nanostructure of the immature and mature BMDCs.

引用

页数：9

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