Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy

被引：29
作者：
Xing, Feiyue [1 ]
Wang, Jiongkun [1 ]
Hu, Mingqian [2 ]
Yu, Yu [3 ,4 ]
Chen, Guoliang [1 ]
Liu, Jing [5 ]
机构：
[1] Jinan Univ, Inst Tissue Transplantat & Immunol, Guangzhou 510632, Guangdong, Peoples R China
[2] Jinan Univ, Dept Chem, Guangzhou 510632, Guangdong, Peoples R China
[3] H Lee Moffitt Canc Ctr & Res Inst, Dept Immunol, Tampa, FL 33612 USA
[4] H Lee Moffitt Canc Ctr & Res Inst, Dept Blood & Marrow Transplantat, Tampa, FL 33612 USA
[5] Jinan Univ, Dept Stomatol, Guangzhou 510632, Guangdong, Peoples R China
来源：
NANOSCALE RESEARCH LETTERS | 2011年 / 6卷
基金：
中国国家自然科学基金;
关键词：
dendritic cell; nanostructure; adhesion force; comparison; GM-CSF; MATURATION; SURVIVAL; AFM; DIFFERENTIATION; KINASE; IL-4; SEM;
D O I：
10.1186/1556-276X-6-455
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
A comparative study of immature and mature bone marrow-derived dendritic cells (BMDCs) was first performed through an atomic force microscope (AFM) to clarify differences of their nanostructure and adhesion force. AFM images revealed that the immature BMDCs treated by granulocyte macrophage-colony stimulating factor plus IL-4 mainly appeared round with smooth surface, whereas the mature BMDCs induced by lipopolysaccharide displayed an irregular shape with numerous pseudopodia or lamellapodia and ruffles on the cell membrane besides becoming larger, flatter, and longer. AFM quantitative analysis further showed that the surface roughness of the mature BMDCs greatly increased and that the adhesion force of them was fourfold more than that of the immature BMDCs. The nano-features of the mature BMDCs were supported by a high level of IL-12 produced from the mature BMDCs and high expression of MHC-II on the surface of them. These findings provide a new insight into the nanostructure of the immature and mature BMDCs.
引用
收藏
页数：9
相关论文
共 36 条
  • [1] Metallic haptens induce differential phenotype of human dendritic cells through activation of mitogen-activated protein kinase and NF-κB pathways
    Antonios, Diane
    Ade, Nadege
    Kerdine-Romer, Saadia
    Assaf-Vandecasteele, Hind
    Larange, Alexandre
    Azouri, Hayat
    Pallardy, Marc
    [J]. TOXICOLOGY IN VITRO, 2009, 23 (02) : 227 - 234
  • [2] PROTEIN IMAGES OBTAINED BY STM, AFM AND TEM
    ARAKAWA, H
    UMEMURA, K
    IKAI, A
    [J]. NATURE, 1992, 358 (6382) : 171 - &
  • [3] The PI3 kinase, p38 SAP kinase, and NF-κB signal transduction pathways are involved in the survival and maturation of lipopolysaccharide-stimulated human monocyte-derived dendritic cells
    Ardeshna, KM
    Pizzey, AR
    Devereaux, S
    Khwaja, A
    [J]. BLOOD, 2000, 96 (03) : 1039 - 1046
  • [4] Dendritic cells and the control of immunity
    Banchereau, J
    Steinman, RM
    [J]. NATURE, 1998, 392 (6673) : 245 - 252
  • [5] Experimental test of blind tip reconstruction for scanning probe microscopy
    Dongmo, LS
    Villarrubia, JS
    Jones, SN
    Renegar, TB
    Postek, M
    Song, JF
    [J]. ULTRAMICROSCOPY, 2000, 85 (03) : 141 - 153
  • [6] Probing molecular recognition sites on biosurfaces using AFM
    Dupres, Vincent
    Verbelen, Claire
    Dufrene, Yves F.
    [J]. BIOMATERIALS, 2007, 28 (15) : 2393 - 2402
  • [7] An advanced approach for the characterization of dendritic cell-induced T cell proliferation in situ
    Florian, Christian
    Barth, Thomas
    Wege, Anja K.
    Maennel, Daniela N.
    Ritter, Uwe
    [J]. IMMUNOBIOLOGY, 2010, 215 (9-10) : 855 - 862
  • [8] Cleaning AFM colloidal probes by mechanically scrubbing with supersharp "brushes"
    Gan, Yang
    Franks, George V.
    [J]. ULTRAMICROSCOPY, 2009, 109 (08) : 1061 - 1065
  • [9] Goldsbury CS, 2009, CURR PROTOC PROTEIN
  • [10] Nanostructure and force spectroscopy analysis of human peripheral blood CD4+ T cells using atomic force microscopy
    Hu, Mingqian
    Wang, Jiongkun
    Cai, Jiye
    Wu, Yangzhe
    Wang, Xiaoping
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 374 (01) : 90 - 94
1234