Study on the interaction of carbaryl annul ctDNA by resonance light scattering spectra and its application

被引：0

作者：

Rao Zhen-hong ^{[1
]}

Liu Shi-lu ^{[1
]}

Sun Ying ^{[1
]}

Min Shun-geng ^{[1
]}

Zhao Hui-yu ^{[1
]}

机构：

[1] China Agr Univ, Coll Sci, Beijing 100094, Peoples R China

来源：

SPECTROSCOPY AND SPECTRAL ANALYSIS | 2008年 / 28卷 / 02期

关键词：

resonance light scattering spectra; carbaryl; ctDNA; interaction; ctDNA determination;

D O I：

暂无

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Interaction modes of carbaryl and DNA were studied by resonance light scattering (RLS) spectra and absorption Spectra Experiments show that at pH 1.97, there are two interaction modes between carbaryl and ctDNA, namely surface assembly mode and intercalative mode. Interaction mode has a relation with the concentration ratio of carbaryl to ctDNA. Under this condition, the RLS intensity of carbaryl and ctDNA is proportional to the concentration of ctDNA. According to this, one new simple and rapid method of determining ctDNA was set up. The linear ranges of the concentration of ctDNA were 0.02-3 mu g center dot mL(-1), the linear regression equation was I=200.77c(mu g center dot mL(-1))+118.91, and the correlation coefficient was r=0.998 9. This method has successfully been used to determine the synthetic samples.

引用

页码：399 / 402

页数：4

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