Antifungal and Antibiofilm In Vitro Activities of Ursolic Acid on Cryptococcus neoformans

被引：8
|
作者：
Wang, Wen-Jing [1 ]
Liu, Chan-Chan [2 ]
Li, Yan-Tong [1 ]
Li, Miao-Qian [1 ]
Fu, Yu-Ting [1 ]
Li, Xin-Chen [1 ]
Jie-Kang [3 ]
Qian, Wei-Dong [1 ,4 ]
机构：
[1] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China
[2] Xian Med Coll, Xian 710309, Peoples R China
[3] Shaanxi Inst Prod Qual Supervis & Inspect, Xian 710048, Peoples R China
[4] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Dept Pharm, Xian 710021, Peoples R China
来源：
CURRENT MICROBIOLOGY | 2022年 / 79卷 / 10期
关键词：
VIRULENCE; CAPSULE; PLANTS;
D O I：
10.1007/s00284-022-02992-5
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Ursolic acid (UA) exists in a variety of medicinal plants. UA exhibits antimicrobial activity against several microorganisms; however, little is known regarding the potential antifungal effect of UA on Cryptococcus neoformans (C. neoformans). The antifungal and antibiofilm activities of UA on C. neoformans H99 were evaluated in this study. Minimum inhibitory concentration (MIC) of UA against C. neoformans H99 was determined by microdilution technique, and its action mode was elucidated by clarifying the variations in cell membrane integrity, capsule, and melanin production. Moreover, the inhibition and dispersal effects of UA on biofilm formation and mature biofilms by C. neoformans H99 were evaluated using crystal violet (CV) assay, optical microscopy, field emission scanning electron microscopy and confocal laser scanning microscopy. The results indicated that the MIC value of UA against C. neoformans H99 was 0.25 mg/mL. UA disrupted the cell membrane integrity, inhibited the capsule and melanin production of C. neoformans H99 in a concentration-dependent manner. Further, UA presented the inhibitory effect on biofilm formation and dispersed mature biofilms, as well as compromised the cell membrane integrity of C. neoformans H99 cells within biofilms. Together, these results indicate that UA might be a potential therapeutic option for the treatment of C. neoformans-related infections.
引用
收藏
页数：9
相关论文
共 50 条
  • [21] A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans
    Zhang, Yang
    Zhou, Liyan
    Liu, Yan
    Zhao, Xi
    Lian, Xianqiang
    Zhang, Jie
    Zhao, De
    Wang, Yujuan
    Zhong, Jin
    Wang, Junfeng
    Wang, Hongli
    Wang, Linqi
    Fu, Yu, V
    MICROBIOLOGY SPECTRUM, 2022, 10 (01):
  • [22] Proteomics reveals that the antifungal activity of fenbendazole against Cryptococcus neoformans requires protein kinases
    de Oliveira, Haroldo C.
    Santos, Marlon D. M.
    Camillo-Andrade, Amanda C.
    Castelli, Rafael F.
    dos Reis, Flavia C. G.
    Carvalho, Paulo C.
    Rodrigues, Marcio L.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2024, 63 (05)
  • [23] Cryptococcus neoformans responds to mannitol by increasing capsule size in vitro and in vivo
    Guimaraes, Allan Jefferson
    Frases, Susana
    Cordero, Radames J. B.
    Nimrichter, Leonardo
    Casadevall, Arturo
    Nosanchuk, Joshua D.
    CELLULAR MICROBIOLOGY, 2010, 12 (06) : 740 - 753
  • [24] Fluconazole induces ROS in Cryptococcus neoformans and contributes to DNA damage in vitro
    Peng, Congyue Annie
    Gaertner, Andrea A. E.
    Henriquez, Sarah Ana
    Fang, Diana
    Colon-Reyes, Rodney J.
    Brumaghim, Julia L.
    Kozubowski, Lukasz
    PLOS ONE, 2018, 13 (12):
  • [25] Molecular Characterization and Antifungal Susceptibility Testing of Sequentially Obtained Clinical Cryptococcus deneoformans and Cryptococcus neoformans Isolates from Ljubljana, Slovenia
    Tomazin, Rok
    Matos, Tadeja
    Meis, Jacques F.
    Hagen, Ferry
    MYCOPATHOLOGIA, 2018, 183 (02) : 371 - 380
  • [26] Synergistic antifungal interaction of N-(butylcarbamothioyl) benzamide and amphotericin B against Cryptococcus neoformans
    Andriani, Gabriella Maria
    de Almeida Spoladori, Lais Fernanda
    Fabris, Marcieli
    Camargo, Priscila Goes
    Lopes Pereira, Patricia Morais
    Santos, Jussevania Pereira
    Bartolomeu-Goncalves, Guilherme
    Alonso, Lais
    Contreras Lancheros, Cesar Armando
    Alonso, Antonio
    Nakamura, Celso Vataru
    Macedo Jr, Fernando
    Pinge-Filho, Phileno
    Yamauchi, Lucy Megumi
    Ferreira Bispo, Marcelle de Lima
    Tavares, Eliandro Reis
    Yamada-Ogatta, Sueli Fumie
    FRONTIERS IN MICROBIOLOGY, 2023, 14
  • [27] Does Cryptococcus neoformans infection alter antifungal distribution: an animal model exploring pharmacokinetic changes
    Alves, Izabel Almeida
    Staudt, Keli Jaqueline
    Torres, Bruna Gaelzer Silva
    Oliveira, Calinca Elioterio
    Rieper, Ryan Lago Araujo
    Kowalski, Layza
    de Araujo, Bibiana Verlindo
    FUTURE MICROBIOLOGY, 2025, 20 (06) : 489 - 498
  • [28] Quantitative evaluation of cryptococcal pathogenesis and antifungal drugs using a silkworm infection model with Cryptococcus neoformans
    Matsumoto, Y.
    Miyazaki, S.
    Fukunaga, D. H.
    Shimizu, K.
    Kawamoto, S.
    Sekimizu, K.
    JOURNAL OF APPLIED MICROBIOLOGY, 2012, 112 (01) : 138 - 146
  • [29] The Role of Amino Acid Permeases and Tryptophan Biosynthesis in Cryptococcus neoformans Survival
    Santos Fernandes, Joao Daniel
    Martho, Kevin
    Tofik, Veridiana
    Vallim, Marcelo A.
    Pascon, Renata C.
    PLOS ONE, 2015, 10 (07):
  • [30] Exploring the antifungal, antibiofilm and antienzymatic potential of Rottlerin in an in vitro and in vivo approach
    Sousa Silva, Nagela Bernadelli
    Menezes, Ralciane Paula
    Goncalves, Daniela Silva
    Santiago, Mariana Brentini
    Conejo, Noemi Chagas
    Souza, Sara Lemes
    Oliveira Santos, Anna Livia
    da Silva, Robinson Sabino
    Ramos, Salvador Boccaletti
    Vieira Ferro, Eloisa Amalia
    Gomes Martins, Carlos Henrique
    SCIENTIFIC REPORTS, 2024, 14 (01):
12345