A validated HPLC-MS/MS method for estimating the concentration of the ganglioside, GD2, in human plasma or serum

被引:15
作者
Busch, C. M. [1 ]
Desai, A. V. [2 ]
Moorthy, G. S. [1 ]
Fox, E. [1 ]
Balis, F. M. [1 ]
机构
[1] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA
[2] Univ Chicago Med & Biol Sci, Chicago, IL USA
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2018年 / 1102卷
关键词
Ganglioside G(D2); Neuroblastoma biomarker; HPLC-MS/MS; THIN-LAYER-CHROMATOGRAPHY; MASS-SPECTROMETRY; GD2; GANGLIOSIDE; LIQUID;
D O I
10.1016/j.jchromb.2018.10.010
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
G(D2) is a ganglioside found in the plasma membrane of the neural crest-derived cancer, neuroblastoma. G(D2) is shed into the circulation of patients with neuroblastoma and could serve as a tumor biomarker to monitor tumor burden or response to treatment. We developed and validated a high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method to quantify the D18:1-18:0 (C18) and the D18:1-20:0 (C20) lipoforms of G(D2) in human plasma and serum. Human brain derived G(D2) containing a mixture of C18 and C20 was used as the analytical standard. Samples were extracted with methanol containing dueterated-G(M1) (internal standard), and analytes were separated on a Phenomenex Kinetex C-18 column eluted with a gradient mobile phase composed of ammonium acetate buffer, methanol and isopropanol. An AB Sciex 4500 QTRAP mass spectrometer in negative ion mode was used to quantify the doubly charged G(D2) C18 and C20 lipoform precursor ions (m/z 836.8 and m/z 850.8) that both yield a product ion of m/z 290.0. The calibration curves were linear from 4-1000 ng/mL and 6-1500 ng/mL for G(D2) C18 and C20 lipoforms respectively. Inter-day and infra-day accuracy were within the acceptable validation range in plasma and serum.
引用
收藏
页码:60 / 65
页数:6
相关论文
共 24 条
[1]   NEW SOLVENT SYSTEM FOR HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF GANGLIOSIDES [J].
ANDO, S ;
WAKI, H ;
KON, K .
JOURNAL OF CHROMATOGRAPHY, 1987, 405 :125-134
[2]   Liquid Chromatography-High-Resolution Mass Spectrometry for Quantitative Analysis of Gangliosides [J].
Fong, Bertram ;
Norris, Carmen ;
Lowe, Edwin ;
McJarrow, Paul .
LIPIDS, 2009, 44 (09) :867-874
[3]   Partial synthesis of ganglioside and lysoganglioside lipoforms as internal standards for MS quantification [J].
Gantner, Martin ;
Schwarzmann, Guenter ;
Sandhoff, Konrad ;
Kolter, Thomas .
JOURNAL OF LIPID RESEARCH, 2014, 55 (12) :2692-2704
[4]   Rapid and sensitive LC-ESI-MS of gangliosides [J].
Garcia, Aldo D. ;
Chavez, Jorge L. ;
Mechref, Yehia .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2014, 947 :1-7
[5]   NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF NON-DERIVATIZED GANGLIOSIDE MIXTURES [J].
GAZZOTTI, G ;
SONNINO, S ;
GHIDONI, R .
JOURNAL OF CHROMATOGRAPHY, 1985, 348 (02) :371-378
[6]  
Harvey DJ, 1999, MASS SPECTROM REV, V18, P349, DOI 10.1002/(SICI)1098-2787(1999)18:6<349::AID-MAS1>3.3.CO
[7]  
2-8
[8]   A facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production [J].
Huang, Feng-Tao ;
Han, Yun-Bin ;
Feng, Yan ;
Yang, Guang-Yu .
JOURNAL OF LIPID RESEARCH, 2015, 56 (09) :1836-1842
[9]   A new liquid chromatography/tandem mass spectrometry method for quantification of gangliosides in human plasma [J].
Huang, Qianyang ;
Zhou, Xiang ;
Liu, Danting ;
Xin, Baozhong ;
Cechner, Karen ;
Wang, Heng ;
Zhou, Aimin .
ANALYTICAL BIOCHEMISTRY, 2014, 455 :26-34
[10]   Targeted analysis of ganglioside and sulfatide molecular species by LC/ESI-MS/MS with theoretically expanded multiple reaction monitoring [J].
Ikeda, Kazutaka ;
Shimizu, Takao ;
Taguchi, Ryo .
JOURNAL OF LIPID RESEARCH, 2008, 49 (12) :2678-2689