Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

被引：159

作者：

Cray, Jonathan A. ^{[1
]}

Stevenson, Andrew ^{[1
]}

Ball, Philip

Bankar, Sandip B. ^{[2
]}

Eleutherio, Elis C. A. ^{[3
]}

Ezeji, Thaddeus C. ^{[4
,5
]}

Singhal, Rekha S. ^{[6
]}

Thevelein, Johan M. ^{[7
,8
]}

Timson, David J. ^{[1
]}

Hallsworth, John E. ^{[1
]}

机构：

[1] Queens Univ Belfast, Sch Biol Sci, Inst Global Food Secur, MBC, Belfast BT9 7BL, Antrim, North Ireland

[2] Bharati Vidyapeeth Univ, Dept Chem Engn, Coll Engn, Pune 411043, Maharashtra, India

[3] Univ Fed Rio de Janeiro, Inst Quim, Programa Posgrad Bioquim, Rio De Janeiro, RJ, Brazil

[4] Ohio State Univ, Dept Anim Sci, Wooster, OH 44691 USA

[5] Ohio State Univ, OARDC, Wooster, OH 44691 USA

[6] Inst Chem Technol, Dept Food Engn & Technol, Mumbai 400019, Maharashtra, India

[7] Katholieke Univ Leuven, Inst Bot & Microbiol, Mol Cell Biol Lab, B-3001 Louvain, Belgium

[8] VIB, Dept Mol Microbiol, B-3001 Louvain, Belgium

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2015年 / 33卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

ACETONE-BUTANOL-ETHANOL; INDUSTRIAL SACCHAROMYCES-CEREVISIAE; ACETOBUTYLICUM ATCC 824; BEIJERINCKII NCIMB 8052; INDUCED WATER-STRESS; CLOSTRIDIUM-BEIJERINCKII; DEGRADATION-PRODUCTS; BIO-ETHANOL; MICROBIAL COMMUNITY; ZYMOMONAS-MOBILIS;

D O I：

10.1016/j.copbio.2015.02.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fermentation products can chaotropically disorder macromolecular systems and induce oxidative stress, thus inhibiting biofuel production. Recently, the chaotropic activities of ethanol, butanol and vanillin have been quantified (5.93, 37.4, 174 kJ kg(-1) M-1 respectively). Use of low temperatures and/or stabilizing (kosmotropic) substances, and other approaches, can reduce, neutralize or circumvent product-chaotropicity. However, there may be limits to the alcohol concentrations that cells can tolerate; e.g. for ethanol tolerance in the most robust Saccharomyces cerevisiae strains, these are close to both the solubility limit (<25%, w/v ethanol) and the water-activity limit of the most xerotolerant strains (0.880). Nevertheless, knowledge-based strategies to mitigate or neutralize chaotropicity could lead to major improvements in rates of product formation and yields, and also therefore in the economics of biofuel production.

引用

页码：228 / 259

页数：32

共 230 条

[11] Response to acetaldehyde stress in the yeast Saccharomyces cerevisiae involves a strain-dependent regulation of several ALD genes and is mediated by the general stress response pathway [J].