Complete genome sequence of a novel bovine hepacivirus from Yunnan, China

被引：8

作者：

Qiang, Xin ^{[1
]}

Shen, Xueying ^{[2
]}

Peng, Honghong ^{[1
,3
]}

Guo, Xiaofang ^{[4
]}

He, Zhihai ^{[5
]}

Yao, Mingguo ^{[5
]}

Fu, Guozhang ^{[2
]}

Cui, Yujun ^{[1
]}

Zhang, Xianglilan ^{[1
]}

Huang, Yong ^{[1
]}

Fan, Hang ^{[1
]}

Du, Chunhong ^{[5
]}

Tong, Yigang ^{[1
,6
]}

Mi, Zhiqiang ^{[1
]}

机构：

[1] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[2] Tengchong Ctr Anim Dis Control & Prevent, Tengchong 679100, Yunnan, Peoples R China

[3] Anhui Med Univ, Hefei 230000, Peoples R China

[4] Yunnan Inst Parasit Dis, Yunnan Prov Key Lab Vector Borne Dis Control & Re, Puer 665000, Yunnan, Peoples R China

[5] Yunnan Inst Endem Dis Control & Prevent, Dali 671000, Yunnan, Peoples R China

[6] Beijing Univ Chem Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn BA, Coll Life Sci & Technol, Beijing 100029, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2020年 / 165卷 / 06期

基金：

中国国家自然科学基金;

关键词：

IDENTIFICATION; TAXONOMY; VIROME; CATTLE;

D O I：

10.1007/s00705-020-04611-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We detected a novel bovine hepacivirus N (HNV) subtype, IME_BovHep_01, in the serum of cattle in Tengchong, Yunnan, China, by high-throughput sequencing. The complete genome of IME_BovHep_01, was sequenced using an Illumina MiSeq sequencer and found to be 8850 nt in length, encoding one hypothetical protein. BLASTn analysis showed that the genome sequence shared similarity with the bovine hepacivirus isolate BovHepV_209/Ger/2014, with 88% query coverage and 70.8% identity. However, the highest similarity was to bovine hepacivirus N strain BRBovHep_RS963, for which only a partial genome sequence is available, with 68% query coverage and 81.5% identity. Sequence comparisons and phylogenetic analysis suggested that IME_BovHep_01 is a novel HNV subtype. Importantly, IME_BovHep_01 is the first member of this new genotype for which the complete genome sequence was determined.

引用

页码：1489 / 1494

页数：6

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