Commentary The new era of quantitative cell imaging-challenges and opportunities

被引：26

作者：

Bagheri, Neda ^{[1
,2
]}

Carpenter, Anne E. ^{[3
]}

Lundberg, Emma ^{[4
]}

Plant, Anne L. ^{[5
]}

Horwitz, Rick ^{[1
]}

机构：

[1] Allen Inst Cell Sci, Seattle, WA 98109 USA

[2] Univ Washington, Biol & Chem Engn, Seattle, WA 98195 USA

[3] Broad Inst Harvard & MIT, Imaging Platform, Cambridge, MA 02142 USA

[4] KTH Royal Inst Technol, Sci Life Lab, Sch Engn Sci Chem Biotechnol & Hlth, Stockholm, Sweden

[5] NIST, Gaithersburg, MD 20899 USA

来源：

MOLECULAR CELL | 2022年 / 82卷 / 02期

基金：

美国国家卫生研究院;

关键词：

FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; BREAKING;

D O I：

10.1016/j.molcel.2021.12.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative optical microscopy-an emerging, transformative approach to single-cell biology-has seen dramatic methodological advancements over the past few years. However, its impact has been hampered by challenges in the areas of data generation, management, and analysis. Here we outline these technical and cultural challenges and provide our perspective on the trajectory of this field, ushering in a new era of quantitative, data-driven microscopy. We also contrast it to the three decades of enormous advances in the field of genomics that have significantly enhanced the reproducibility and wider adoption of a plethora of genomic approaches.

引用

页码：241 / 247

页数：7

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