Overexpression of cdk4 and p16 in oral lichen planus supports the concept of premalignancy

被引：27

作者：

Poomsawat, Sopee ^{[2
]}

Buajeeb, Waranun ^{[1
]}

Khovidhunkit, Siribang-on Piboonniyom ^{[3
]}

Punyasingh, Jirapa ^{[2
]}

机构：

[1] Mahidol Univ, Dept Oral Med, Fac Dent, Bangkok 10400, Thailand

[2] Mahidol Univ, Dept Oral Pathol, Fac Dent, Bangkok 10400, Thailand

[3] Mahidol Univ, Dept Hosp Dent, Fac Dent, Bangkok 10400, Thailand

来源：

JOURNAL OF ORAL PATHOLOGY & MEDICINE | 2011年 / 40卷 / 04期

关键词：

cdk4; cdk6; immunohistochemistry; oral lichen planus; p16;

D O I：

10.1111/j.1600-0714.2010.01001.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Background: Cell cycle arrest and increased cell proliferation have been demonstrated in oral lichen planus (OLP). This study evaluated the expression of cdk4, cdk6 and p16, important proteins in the G1 phase, in OLP and compared the expression of these proteins of OLP with those of normal mucosa. Methods: Expression of cdk4, cdk6 and p16 were investigated in 23 OLP and 10 normal mucosae using immunohistochemistry technique. Positive cells were counted and presented as a percentage of positive cells. Results: Expression of cdk4, cdk6 and p16 was observed in 3/10 (30%), 1/10 (10%) and none of normal mucosa, respectively. Expression of cdk4, cdk6 and p16 was detected in 18/23 (78.3%), 8/23 (34.8%) and 15/23 (65.2%), of OLP, respectively. The numbers of cdk4 and p16 positive cases of OLP were significantly higher than normal mucosa. In normal mucosa, the averages of the percentage of positive cells for cdk4 and cdk6 staining were 1.48 and 0.18, respectively. In OLP, the averages of the percentage of positive cells for cdk4, cdk6 and p16 staining were 2.73, 1.06 and 2.24, respectively. The percentage of cdk4-positive cells of OLP was significantly higher than those of normal mucosa group. Conclusion: Oral lichen planus demonstrated overexpression of cdk4 and p16, but not cdk6, suggesting that epithelial cells in OLP are in the hyperproliferative state and in cell arrest. Altered expression of cdk4 and p16 provides evidence of the malignant potential in OLP.

引用

页码：294 / 299

页数：6

共 37 条

[1]

Walsh L.J., Savage N.W., Ishii T., Seymour G.J., Immunopathogenesis of oral lichen planus, J Oral Pathol Med, 19, pp. 389-396, (1990)

[2]

van der Meij E.H., van der Waal I., Lack of clinicopathologic correlation in the diagnosis of oral lichen planus based on the presently available diagnostic criteria and suggestions for modifications, J Oral Pathol Med, 32, pp. 507-512, (2003)

[3]

Bloor B.K., Malik F.K., Odell E.W., Morgan P.R., Quantitative assessment of apoptosis in oral lichen planus, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 88, pp. 187-195, (1999)

[4]

Bascones-Ilundain C., Gonzalez-Moles M.A., Esparza G., Gil-Montoya J.A., Bascones-Martinez A., Significance of liquefaction degeneration in oral lichen planus: a study of its relationship with apoptosis and cell cycle arrest markers, Clin Exp Dermatol, 32, pp. 556-563, (2007)

[5]

Gonzalez-Moles M.A., Bascones-Ilundain C., Gil Montoya J.A., Ruiz-Avila I., Delgado-Rodriguez M., Bascones-Martinez A., Cell cycle regulating mechanisms in oral lichen planus: molecular bases in epithelium predisposed to malignant transformation, Arch Oral Biol, 51, pp. 1093-1103, (2006)

[6]

Bascones C., Gonzalez-Moles M.A., Esparza G., Et al., Apoptosis and cell cycle arrest in oral lichen planus Hypothesis on their possible influence on its malignant transformation, Arch Oral Biol, 50, pp. 873-881, (2005)

[7]

da Silva Fonseca L.M., do Carmo M.A., Identification of the AgNORs, PCNA and ck16 proteins in oral lichen planus lesions, Oral Dis, 7, pp. 344-348, (2001)

[8]

Hirota M., Ito T., Okudela K., Et al., Cell proliferation activity and the expression of cell cycle regulatory proteins in oral lichen planus, J Oral Pathol Med, 31, pp. 204-212, (2002)

[9]

Taniguchi Y., Nagao T., Maeda H., Kameyama Y., Warnakulasuriya K.A., Epithelial cell proliferation in oral lichen planus, Cell Prolif, 35, SUPPL. 1, pp. 103-109, (2002)

[10]

Mattsson U., Jontell M., Holmstrup P., Oral lichen planus and malignant transformation: is a recall of patients justified?, Crit Rev Oral Biol Med, 13, pp. 390-396, (2002)

← 1 2 3 4 →