An ultrasensitive and visible lighting-up probe for imaging thiophenols in water samples, in serum and visualizing thiophenols-induced oxidative stress process in live cells

被引：7

作者：

Feng, Yan ^{[1
,2
]}

Cao, Chen ^{[1
,2
]}

Ru, Jiaxi ^{[3
,4
]}

Yang, Yang ^{[1
,2
]}

Wang, Yingzhe ^{[1
,2
]}

Song, Xuerui ^{[1
,2
]}

Wang, Kun ^{[1
,2
]}

Zhang, Guolin ^{[1
,2
]}

Liu, Weisheng ^{[1
,2
]}

机构：

[1] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China

[2] Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ, Lanzhou 730000, Peoples R China

[3] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Minist Agr, State Key Lab Vet Etiol Biol, Xujiaping 1, Lanzhou 730046, Gansu, Peoples R China

[4] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Minist Agr, Key Lab Anim Virol, Xujiaping 1, Lanzhou 730046, Gansu, Peoples R China

来源：

TALANTA | 2020年 / 210卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Thiophenols; Water-soluble; Oxidative stress; Actual water sample; Biological samples; RATIOMETRIC FLUORESCENT-PROBE; SENSITIVE DETECTION; SELECTIVE DETECTION; AQUEOUS-SOLUTION; LIVING CELLS; COLORIMETRIC CHEMOSENSORS; ENVIRONMENTAL WATER; HYDROGEN-PEROXIDE; TOXIC THIOPHENOLS; REACTIVE OXYGEN;

D O I：

10.1016/j.talanta.2019.120622

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Thiophenols, a class of significant industrial materials, are extremely toxic in environmental as well live cells. However, the process of live cells responding to thiophenols is not well understood. Herein, an innovative "OFF-ON" probe FY for thiophenols selectively in 100% aqueous solution was reported. It featured rapid response (similar to 150 s), prominent sensitivity (detection limit: 5 nM), and large Stokes shift (similar to 104 nm), which assured specific detection of thiophenols in A375 cells, HeLa cells and environment. Especially, it proved that thiophenols in live cells can be eliminated by endogenous reactive oxygen species (ROS), indicating that thiophenols may result in cellular oxidative stress. As well, it was resoundingly put into recognizing of thiophenols quantitatively in actual water samples and in serum.

引用

页数：12

共 54 条

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