Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: A metabolomic approach

被引：26

作者：

Longini, M. ^{[1
]}

Giglio, S. ^{[1
]}

Perrone, S. ^{[1
]}

Vivi, A. ^{[1
]}

Tassini, M. ^{[1
]}

Fanos, V. ^{[2
,3
]}

Sarafidis, K. ^{[4
]}

Buonocore, G. ^{[1
]}

机构：

[1] Univ Siena, Dept Mol & Dev Med, I-53100 Siena, Italy

[2] Univ Cagliari, Neonatal Intens Care Unit, Inst Puericulture, I-09124 Cagliari, Italy

[3] Univ Cagliari, Neonatal Sect, I-09124 Cagliari, Italy

[4] Aristotle Univ Thessaloniki, Fac Med, Dept Neonatol 1, GR-54006 Thessaloniki, Greece

来源：

CLINICA CHIMICA ACTA | 2015年 / 444卷

关键词：

H-1; NMR; Metabolomics; Urine; Asphyxia; NMR; PRIORITIES; SELECTION; TOOL; PLS;

D O I：

10.1016/j.cca.2015.02.004

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

In order to highlight differences in the metabolic profile of healthy (control) compared with asphyxiated newborns, by using untargeted metabolomic approach coupled with H-1 NMR spectroscopy, we evaluated the effects of asphyxia on newborn urine metabolites. Our results showed that lactate, glucose and TMAO, together with threonine plus 3-hydroxyisovalerate are the metabolites more characterizing the asphyxiated group; lower contribute to discrimination is related to other metabolites such as dimethylglycine, dimethylamine, creatine, succinate, formate, urea and aconitate. After 24-48 h from resuscitation preterm asphyctic neonates showed their recovery pattern that still can be differentiated by the controls. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：250 / 256

页数：7

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