Micropropagation methods of pineapple

The objective of this work was to compare different methods of pineapple in vitro mass micropropagation. The treatments were conventional micropropagation in solid medium ( 5 and 6 g L-1 agar) and in liquid medium, micropropagation in bioreactor of temporary immersion ( immersion in culture medium each 2, 4 and 8 hours, for 3 minutes) and continuous immersion ( with aeration each 2, 4 and 8 hours, for 3 minutes). MS medium supplement with 1 mg L-1 BAP, 0.25 mg L-1 NAA, and pH adjusted to 5.8 was utilized. Cultures were incubated at 25 +/- 1 degrees C under cool white fluorescent tubes ( 40 mu mol m(-2) s(-1)) with a 16-hours photoperiod. The experiment design was completely randomized, comprising nine treatments and four replicates. After 45 days, number of shootings, shootings higher than 1 cm, length of shootings, fresh matter of shootings and dry matter of shootings were evaluated. Temporary immersion system in each 2 hours for 3 minutes provided higher number, height and dry matter of pineapple shootings. Temporary immersion system is the most efficient method in pineapple mass micropropagation.