Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase

被引:109
作者
Lee, Shoken [1 ]
Uchida, Yasunori [1 ]
Wang, Jiao [2 ,3 ]
Matsudaira, Tatsuyuki [1 ]
Nakagawa, Takatoshi [4 ]
Kishimoto, Takuma [5 ]
Mukai, Kojiro [1 ,5 ]
Inaba, Takehiko [5 ]
Kobayashi, Toshihide [5 ]
Molday, Robert S. [2 ,3 ]
Taguchi, Tomohiko [1 ,6 ]
Arai, Hiroyuki [1 ,6 ]
机构
[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Hlth Chem, Tokyo, Japan
[2] Univ British Columbia, Ctr Macular Res, Dept Biochem & Mol Biol, Vancouver, BC V5Z 1M9, Canada
[3] Univ British Columbia, Ctr Macular Res, Dept Ophthalmol & Visual Sci, Vancouver, BC V5Z 1M9, Canada
[4] Osaka Med Coll, Dept Pharmacol, Takatsuki, Osaka 569, Japan
[5] RIKEN, Lipid Biol Lab, Wako, Saitama, Japan
[6] Univ Tokyo, Grad Sch Pharmaceut Sci, Pathol Cell Biol Lab, Tokyo, Japan
基金
美国国家卫生研究院; 加拿大健康研究院;
关键词
ATP8A1; EHD1; phosphatidylserine; phospholipid flippase; recycling endosomes; DENSITY LIPOPROTEIN RECEPTOR; PUTATIVE AMINOPHOSPHOLIPID TRANSLOCASE; P-TYPE ATPASES; SUBCELLULAR-LOCALIZATION; QUADRUPEDAL LOCOMOTION; CEREBELLAR HYPOPLASIA; PLASMA-MEMBRANE; BETA-SUBUNIT; PROTEIN; COMPARTMENT;
D O I
10.15252/embj.201489703
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
P-4-ATPases translocate aminophospholipids, such as phosphatidylserine (PS), to the cytosolic leaflet of membranes. PS is highly enriched in recycling endosomes (REs) and is essential for endosomal membrane traffic. Here, we show that PS flipping by an RE-localized P-4-ATPase is required for the recruitment of the membrane fission protein EHD1. Depletion of ATP8A1 impaired the asymmetric transbilayer distribution of PS in REs, dissociated EHD1 from REs, and generated aberrant endosomal tubules that appear resistant to fission. EHD1 did not show membrane localization in cells defective in PS synthesis. ATP8A2, a tissue-specific ATP8A1 paralogue, is associated with a neurodegenerative disease (CAMRQ). ATP8A2, but not the disease-causative ATP8A2 mutant, rescued the endosomal defects in ATP8A1-depleted cells. Primary neurons from Atp8a2(-/-) mice showed a reduced level of transferrin receptors at the cell surface compared to Atp8a2(+/+) mice. These findings demonstrate the role of P4-ATPase in membrane fission and give insight into the molecular basis of CAMRQ.
引用
收藏
页码:669 / 688
页数:20
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