Supercritical Carbon Dioxide Extraction of Astaxanthin, Lutein, and Fatty Acids from Haematococcus pluvialis Microalgae

被引:93
作者
Di Sanzo, Giuseppe [1 ]
Mehariya, Sanjeet [2 ,3 ]
Martino, Maria [1 ]
Larocca, Vincenzo [1 ]
Casella, Patrizia [2 ]
Chianese, Simeone [3 ]
Musmarra, Dino [3 ]
Balducchi, Roberto [1 ]
Molino, Antonio [2 ]
机构
[1] ENEA, Italian Natl Agcy New Technol Energy & Sustainabl, Dept Sustainabil, CR Trisaia SS Jonica 106,Km 419 500, I-75026 Rotondella, Italy
[2] ENEA, Italian Natl Agcy New Technol Energy & Sustainabl, Dept Sustainabil, CR Portici P Enrico Fermi 1, I-80055 Portici, Italy
[3] Univ Campania L Vanvitelli, Dept Engn, Via Roma 29, I-81031 Aversa, Italy
基金
欧盟地平线“2020”;
关键词
microalgae; Haematococcus pluvialis; astaxanthin; lutein; fatty acids; supercritical fluid extraction; natural medicines; CO2; EXTRACTION; FLUID EXTRACTION; BIOACTIVE COMPOUNDS; PHARMACEUTICAL IMPORTANCE; COMMERCIAL APPLICATIONS; VEGETABLE-OIL; SCENEDESMUS; CAROTENOIDS; RECOVERY; ETHANOL;
D O I
10.3390/md16090334
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Haematococcus pluvialis microalgae in the red phase can produce significant amounts of astaxanthin, lutein, and fatty acids (FAs), which are valuable antioxidants in nutraceutics and cosmetics. Extraction of astaxanthin, lutein, and FAs from disrupted biomass of the H. pluvialis red phase using carbon dioxide (CO2) in supercritical fluid extraction (SFE) conditions was investigated using a bench-scale reactor in a semi-batch configuration. In particular, the effect of extraction time (20, 40, 60, 80, and 120 min), CO2 flow rate (3.62 and 14.48 g/min) temperature (50, 65, and 80 degrees C), and pressure (100, 400, and 550 bar.) was explored. The results show the maximum recovery of astaxanthin and lutein achieved were 98.6% and 52.3%, respectively, at 50 degrees C and 550 bars, while the maximum recovery of FAs attained was 93.2% at 65 degrees C and 550 bars.
引用
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页数:18
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