Eriodictyol inhibits IL-1β-induced inflammatory response in human osteoarthritis chondrocytes

被引：48
作者：
Wang, Yongsheng [1 ]
Chen, You [1 ]
Chen, Ying [2 ]
Zhou, Bingkang [1 ]
Shan, Xiaowei [1 ]
Yang, Guangjie [1 ]
机构：
[1] Henan Univ, Dept Orthoped, Affiliated Hosp 1, 357 Ximen St, Kaifeng 475000, Henan, Peoples R China
[2] Henan Univ, Dept Anesthesiol, Affiliated Hosp 1, Kaifeng 475000, Henan, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2018年 / 107卷
关键词：
Osteoarthritis (OA); Inflammatory response; Eriodictyol; Chondrocytes; NF-kappa B; Nrf2/HO-1; signaling; NF-KAPPA-B; ACTIVATION; EXPRESSION; GENETICS;
D O I：
10.1016/j.biopha.2018.08.103
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Osteoarthritis (OA) is a degenerative disease of joints, which is closely associated with cartilage degradation. Eriodictyol, a natural flavonoid compound, has been reported to have anti-inflammatory and anti-osteoclastogenic effects. However, the effect of eriodictyol on inflammatory response in OA has not been investigated. Our results showed that eriodictyol attenuated the inhibition of cell viability in IL-1 beta-stimulated chondrocytes. In addition, eriodictyol inhibited the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of prostaglandin E2 (PGE2) and nitric oxide (NO), which were induced by IL-1 beta. The induction of inflammatory cytokines and matrix metalloproteinases (MMPs) caused by IL-1 beta stimulation was also attenuated by eriodictyol. Furthermore, eriodictyol pretreatment inhibited I kappa B alpha degradation and the level of p-p65, and enhanced the up-regulation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase 1 (HO-1) in IL-1 beta-stimulated chondrocytes. Si-Nrf2 treatment significantly inhibited the expressions of Nrf2 and HO-1 in chondrocytes. Additionally, si-Nrf2 transfection also abolished the anti-inflammatory effects of eriodictyol in chondrocytes. These findings indicated that eriodictyol exhibited anti-inflammatory effect in IL1 beta-stimulated chondrocytes. The effect was mediated by inhibiting NF-kappa B via activating the Nrf2/HO-1 signaling pathway.
引用
收藏
页码：1128 / 1134
页数：7
相关论文
共 23 条
[1]   The effect of IL-1β on the expression of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in human chondrocytes [J].
Aida, Y ;
Maeno, M ;
Suzuki, N ;
Shiratsuchi, H ;
Motohashi, M ;
Matsumura, H .
LIFE SCIENCES, 2005, 77 (25) :3210-3221
[2]   Inhibition of cartilage degradation and suppression of PGE2 and MMPs expression by pomegranate fruit extract in a model of posttraumatic osteoarthritis [J].
Akhtar, Nahid ;
Khan, Nazir M. ;
Ashruf, Omer S. ;
Haqqi, Tariq M. .
NUTRITION, 2017, 33 :1-13
[3]   Osteoarthritis as an inflammatory disease (osteoarthritis is not osteoarthrosis!) [J].
Berenbaum, F. .
OSTEOARTHRITIS AND CARTILAGE, 2013, 21 (01) :16-21
[4]   MiR-149 suppresses the inflammatory response of chondrocytes in osteoarthritis by down-regulating the activation of TAK1/NF-κB [J].
Chen, Qiaojie ;
Wu, Shaokun ;
Wu, Yaojun ;
Chen, Liang ;
Pang, Qingjiang .
BIOMEDICINE & PHARMACOTHERAPY, 2018, 101 :763-768
[5]   Pro- and Antioxidant Activity of Three Selected Flavan Type Flavonoids: Catechin, Eriodictyol and Taxifolin [J].
Chobot, Vladimir ;
Hadacek, Franz ;
Bachmann, Gert ;
Weckwerth, Wolfram ;
Kubicova, Lenka .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (12)
[6]   A clinical perspective of IL-1β as the gatekeeper of inflammation [J].
Dinarello, Charles A. .
EUROPEAN JOURNAL OF IMMUNOLOGY, 2011, 41 (05) :1203-1217
[7]   Nrf2 mediates redox adaptations to exercise [J].
Done, Aaron J. ;
Traustadottir, Tinna .
REDOX BIOLOGY, 2016, 10 :191-199
[8]   Osteoarthritis [J].
Glyn-Jones, S. ;
Palmer, A. J. R. ;
Agricola, R. ;
Price, A. J. ;
Vincent, T. L. ;
Weinans, H. ;
Carr, A. J. .
LANCET, 2015, 386 (9991) :376-387
[9]   The role of IL-1 and IL-1RA in joint inflammation and cartilage degradation [J].
Jacques, Claire ;
Gosset, Marjolaine ;
Berenbaum, Francis ;
Gabay, Cem .
INTERLEUKINS, 2006, 74 :371-403
[10]   Anti-Inflammatory Effects of Licochalcone A on IL-1β-Stimulated Human Osteoarthritis Chondrocytes [J].
Jia, Tao ;
Qiao, Jiutao ;
Guan, Dehong ;
Chen, Tianxin .
INFLAMMATION, 2017, 40 (06) :1894-1902
123