Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum

被引：29

作者：

Macis, L ^{[1
]}

Daniel, R ^{[1
]}

Gottschalk, G ^{[1
]}

机构：

[1] Univ Gottingen, Inst Mikrobiol & Genet, D-37077 Gottingen, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 164卷 / 01期

关键词：

Clostridium pasteurianum; coenzyme B-12; 1,3-propanediol; glycerol fermentation; glycerol dehydratase;

D O I：

10.1111/j.1574-6968.1998.tb13062.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genes encoding coenzyme B-12-dependent glycerol dehydratase of Clos:Clostridium pasteurianum were subcloned and expressed in Escherichia coli. The native molecular mass of the enzyme is 190 000 Da. The enzyme converts glycerol, 1,2-propanediol and 1.2-ethanediol to 3-hydroxypropionaldehyde, propionaldehyde and acetaldehyde, respectively, but glycerol is the preferred substrate. The nucleotide sequences of the dhaBCE genes encoding the three subunits of glycerol dehydratase and of orfZ whose function is unknown were determined. The deduced products of the dhaBCE genes with calculated molecular masses of 60 813. 19 549 and 16 722 Da, respectively, revealed high similarity to amino acid sequences of subunits of coenzyme B-12-dependent glycerol and diol dehydratases from other organisms. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：21 / 28

页数：8

共 25 条

[11]

HOMANN T, 1990, APPL MICROBIOL BIOT, V33, P121

[12] ON THE PERMEABILITY TO WEAK ACIDS AND BASES OF THE CYTOPLASMIC MEMBRANE OF CLOSTRIDIUM-PASTEURIANUM [J].

KELL, DB ;

PECK, MW ;

RODGER, G ;

MORRIS, JG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1981, 99 (01) :81-88

[13] Glycerol conversion to 1,3-propanediol by Clostridium pasteurianum: cloning and expression of the gene encoding 1,3-propanediol dehydrogenase [J].

Luers, F ;

Seyfried, M ;

Daniel, R ;

Gottschalk, G .

FEMS MICROBIOLOGY LETTERS, 1997, 154 (02) :337-345

[14] Characterization, sequencing, and expression of the genes encoding a reactivating factor for glycerol-inactivated adenosylcobalamin-dependent diol dehydratase [J].

Mori, K ;

Tobimatsu, T ;

Hara, T ;

Toraya, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (51) :32034-32041

[15] COENZYME-B-12 DEPENDENT DIOL DEHYDRASE - PURIFICATION, SUBUNIT HETEROGENEITY, AND REVERSIBLE ASSOCIATION [J].

POZNANSKAJA, AA ;

TANIZAWA, K ;

SODA, K ;

TORAYA, T ;

FUKUI, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1979, 194 (02) :379-386

[16] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[17]

SCHNEIDE.Z, 1970, J BIOL CHEM, V245, P3388

[18] Cloning, sequencing, and overexpression of the genes encoding coenzyme B-12-dependent glycerol dehydratase of Citrobacter freundii [J].

Seyfried, M ;

Daniel, R ;

Gottschalk, G .

JOURNAL OF BACTERIOLOGY, 1996, 178 (19) :5793-5796

[19]

SEYFRIED M, 1997, MOLEKULARBIOLOGISCHE

[20]

SPRENGER GA, 1989, J GEN MICROBIOL, V135, P1255

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