Glycosylation analysis of glycoproteins and proteoglycans using capillary electrophoresis-mass spectrometry strategies

被引：68

作者：

Amon, Sabine ^{[1
]}

Zamfir, Alina D. ^{[2
,3
]}

Rizzi, Andreas ^{[1
]}

机构：

[1] Univ Vienna, Inst Analyt Chem & Food Chem, A-1090 Vienna, Austria

[2] Aurel Vlaicu Univ Arad, Dept Chem & Biol Sci, Arad, Romania

[3] Natl Inst Res & Dev Electrochem & Condensed Matte, Mass Spectrometry Lab, Timisoara, Romania

来源：

ELECTROPHORESIS | 2008年 / 29卷 / 12期

关键词：

capillary electrophoresis; glycopepticles; glycoproteins; glycosylation; mass spectrometry; proteoglycans;

D O I：

10.1002/elps.200800105

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review highlights recent developments in glycosylation analysis by modem MS in combination with CE based preseparation. Focused on CE-MS strategies aimed for glycotyping, the review addresses the detailed glycoform. analysis of glycoproteins, glycopeptides, and proteoglycans. Glycoform. analysis in the context of modem glycoproteomics is briefly addressed, as well. CZE, CIEF, and,frontal analysis CE approaches hyphenated to high-resolution multistage MS for the detailed analysis of protein-linked glycan structures are overviewed in a comprehensive way. Advantages and limitations of various methodological approaches and techniques as well as mass spectrometric instrumentation are discussed in the particular context of glycoanalysis.

引用

页码：2485 / 2507

页数：23

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