Adjuvant Formulation for Companion Animals Vaccines

被引：8

作者：

Deville, S. ^{[1
]}

Carneaux, E. ^{[1
]}

Bertrand, F. ^{[1
]}

Cauchard, S. ^{[2
]}

Cauchard, J. ^{[2
]}

Dupuis, L. ^{[1
]}

机构：

[1] ASEPPIC Tour Kupka C, 7 Blvd Franck Kupka, F-92039 Paris, France

[2] Anses, Lab Pathol Equine Dozule, Maisons Alfort, France

来源：

4TH VACCINE AND ISV ANNUAL GLOBAL CONGRESS | 2011年 / 4卷

关键词：

Adjuvant; Montanide (TM) Pet Gel A; Veterinary adjuvant; pets vaccines; Safety; ALUMINUM ADJUVANTS; MECHANISMS; SAFETY;

D O I：

10.1016/j.provac.2011.07.015

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Companion animals are sensitive species able to strongly react to vaccine. Compared to farm animals, owner's sensibility to vaccine safety is exacerbated due to emotional links between animal and owner. Adjuvant selection during vaccine development is a key parameter driving vaccine safety and efficacy profile. Our studies demonstrated the ability to use Montanide (TM) Pet Gel A (polymeric adjuvant manufactured under GMP rules) in cat, dog and horse vaccines. Adjuvants performances were highlighted by local and general safety parameters but also through vaccine efficacy to trigger a protective immune response against the pathogen. Three trials were performed to validate Montanide (TM) Pet Gel A compatibility with cats, dogs and horses vaccine models. Experimental vaccines were formulated using different antigens according to the animal: inactivated Rhodococcus equi (horse), purified ovalbumin (cat) Leptospira Icterohaemorrhagiae (dogs). In all trials, safety was followed through behavior and temperature measurement. Furthermore, in dog and cat models, histology studies were performed to assess the local reaction in the injection site. A kinetic of blood sampling was performed in all trials. Antigen specific ELISA was used to assess the immune response induced. In cat and dog trials, aluminium based formulation were used as benchmark for Montanide (TM) formulation while in horse we compare Montanide (TM) Pet Gel Abased vaccine to an already published internal reference. Safety performances of Montanide (TM) Pet GelA were superior to aluminium based vaccines in dogs and cats. Transient oedemas were observed in horse vaccine model after each vaccine injection, nevertheless, no impact on the animal behavior was observed. The antibodies production induced by Montanide (TM) Pet Gel Abased vaccines was higher than aluminium based vaccines orinternal reference. Montanide (TM) Pet Gel A can be used associated with a wide range of antigenic media and recommended to be used as adjuvant for sensitive animal's vaccines. (C) 2011 Published by Elsevier Ltd. Selection and peer-review under responsibility of Prof. Ray Spier

引用

页码：104 / 112

页数：9

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