Estimation of the prevalence of Mycobacterium avium subsp paratuberculosis by PCR in sheep blood

被引：12
作者：
Coelho, A. C. [1 ]
Pinto, M. L. [1 ]
Coelho, A. M. [2 ]
Rodrigues, J. [1 ]
Juste, R. [3 ]
机构：
[1] Univ Tras os Montes & Alto Douro, Dept Ciencias Vet, P-5001911 Vila Real Codex, Portugal
[2] Direccao Reg Agr Tras os Montes, P-5370347 Mirandela, Portugal
[3] NEIKER, Inst Vasco Invest & Desarrollo Agr, Dept Sanidad Anim, Derio, Bizcaya, Spain
来源：
SMALL RUMINANT RESEARCH | 2008年 / 76卷 / 03期
关键词：
Mycobacterium avium subsp paratuberculosis; ovine; prevalence; PCR; blood;
D O I：
10.1016/j.smallrumres.2007.12.003
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The prevalence of Mycobacterium avium subsp. paratuberculosis (Map) infection in sheep was estimated by blood PCR. One thousand and five hundred sheep belonging to 150 sheep flocks of Tras-os-Montes e Alto Douro (TMAD, Portugal) were studied. Animals were divided into two groups of five sheep each according their health status: "apparently healthy animals" and "paratuberculosis suspect animals". Samples were pooled in groups of five. PCR detected Map DNA in 56 (18.7%) pool samples. The overall individual prevalence of Map in this study ranged from 6.4% to 15.4%. Map was found in 20.7% pooled samples from apparently healthy and in 16.7% pooled samples from suspect animals. The aim of the present study was to estimate the prevalence of ovine paratuberculosis using a PCR pool screen approach. The results showed that a simple blood PCR method could be reliably performed on pools of five samples in order to estimate the prevalence of Map infection in the ovine population. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：201 / 206
页数：6
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